天津中医药Issue(2)：110-112,3.DOI:10.11656/j.issn.1672-1519.2015.02.12
HS-SpME-GC/MS法分析连花清瘟胶囊制剂原料中的挥发性成分
Determining volatiles from raw materials of Lianhua Qingwen PreParations by HS-SpME-GC/MS
摘要
Abstract
Objective] To analyze the volatiles from raw materials of Lianhua Qingwen preparations. [Methods] Headspace solid-phase micro-extraction (HS-SPME) technique was employed to extract the volatile compounds from raw materials of Lianhua Qingwen preparations. The volatile components were isolated and identified successfully by gas chromatography/mass spectrometry (GC/MS) and matched with NIST05 mass spectra library to retrieve the unknown chemical components automatically, the relative contents in percentage of each chemical composition were calculated by peak area normalization method. [Results] Fifty-three volatile compounds were temporarily identified, corresponding to 98.30%of total oils. The major volatile component was l-menthol (97.87%), and the other high levels of ingredients were patchouli alcohol (1.101%),α-bulnesene (0.398%),α-gurjunene (0.256%),α-guaiene (0.210%),α-patchoulene (0.171%) andβ-patchoulene (0.111%). [Conclusion] The volatile components from raw materials of Lianhua Qingwen preparations can be simply, rapidly and accurately analyzed by HS-SPME-GC/MS method.关键词
连花清瘟胶囊/顶空固相微萃取/气质联用分析/挥发性成分Key words
Lianhua Qingwen preparation/SPME/GC/MS/volatile constituent分类
医药卫生引用本文复制引用
贾伟娜,王春华,王宏涛,田书彦,韩雪,朱彦..HS-SpME-GC/MS法分析连花清瘟胶囊制剂原料中的挥发性成分[J].天津中医药,2015,(2):110-112,3.基金项目
天津市自然科学基金资助项目（13JCZD-JC28600、12JCYBJC32700）。 （）
