生态环境学报Issue(4)：679-684,6.
丁草胺污染对高产水稻土微生物区系的影响
Effects of butachlor-contamination on population of microbial communities in high-yield paddy soils
摘要
Abstract
Soil microorganism is the most potential indicator of soil quality evaluation, and is closely related to the stability and productivity of agricultural ecosystem. Taoyuan, Yunnan province is a special eco-site which keeps the highest yield records of rice cultivation in small planting areas, and butachlor is applied at the pre-emergence stage of rice year in and year out in Taoyuan. Therefore, it is significant to understand the ecological effects of the herbicide butachlor on microbial communities in high-yield paddy soils. Effects of butachlor-contamination on population of aerobic bacteria, actinobacteria, fungi, nitrogen fixing bacteria、phosphate solubilizing bacteria and silicate dissolving bacteria in high-yield paddy soils were investigated under laboratory conditions, and the microbial communities structures under different butachlor concentrations (calculated by active ingredient 0.15, 0.30 and 1.5 mg·kg-1) were evaluated using plate count. The results showed that the number of aerobic bacteria were higher than CK 78.6% and 153.8% after application of butachlor at high or middle concentrations (0.30 and 1.50 mg/kg dried soil) 7d later, and then declined significantly, while the number did not change significantly at low butachlor concentration (0.15 mg·kg-1 dried soil). The number of actinomycetes was higher than CK 75.1% after application of butachlor at high concentrations 7 d later, and 125.0% at middle concentrations 15 d later, butachlor at high or middle concentrations accelerated the growth of actinomycetes, while showed an inhibition at low concentration. The proliferation and growth of fungi did not change significantly at low butachlor concentration, while stimulated first and then restrained at middle butachlor concentration, and showed a sustained inhibition at high butachlor concentration. The number of fungi was higher than CK 56.9% after application of butachlor at middle concentrations 30 d later, and the number were lower than CK at high concentrations throughout. Butachlor at all concentrations accelerated the growth of nitrogen fixing bacteria, however, with the prolongation of culture time, application of butachlor at high concentration showed an inhibition. The number of nitrogen fixing bacteria were higher than CK 237.1%, 179.9% and 138.1% after application of butachlor at low, middle and high concentrations 7 d later. Butachlor at all concentrations restrained the proliferation and growth of phosphate solubilizing bacteria, and the inhibitory action is stronger with the concentration increased. The number of silicate dissolving bacteria did not change significantly at low butachlor concentration, while showed a strong inhibition at high butachlor concentration. The number of silicate dissolving bacteria was lower than CK 60.7% 7 d later and 58.1% 15 d later after application of butachlor at high concentrations.关键词
丁草胺/高产水稻土/微生物区系/自生固氮菌/磷酸盐溶解菌/硅酸盐溶解菌Key words
butachlor/high-yield paddy soil/microbial communities/nitrogen-fixing bacteria/phosphate solubilizing bacteria/silicate dissolving bacteria分类
资源环境引用本文复制引用
张仕颖,夏运生,肖炜,崔晓龙,王永霞,史静,张乃明..丁草胺污染对高产水稻土微生物区系的影响[J].生态环境学报,2014,(4):679-684,6.基金项目
国家自然科学基金项目 （）
