生态环境学报Issue(8)：1272-1279,8.
平顶山、长白山、赛罕乌拉森林土壤微生物群落结构分析
The Microbial Community of Three Mountains Pingding, Changbai and Saihanwula Based on the Analysis of Phospholipid Fatty Acids(PLFAs)
摘要
Abstract
Soil microbes is the key and motivation of nutrient and energy cycling in terrestrial ecosystem. It is hard to evaluate the community structure and ecological functions of soil ecosystem for its abundant diversity. Phospholipid fatty acid (PLFA), a relatively stable and heritable biomarker of live microorganism, which can be used to indicate the exist and situation of specific organism or population, has been widely used in elucidating the dynamic change of microbial community in soil. The present study evaluated soil microbial biomass, Bacteria, Fungus, Gram+ Bacteria and Gram- Bacteria composition in typical forest soils of Chinese northeast area (including Pingding Mountain, Changbai Mountain and Saihanwula Mountain) with the analysis of phospholipid fatty acid (PLFA). Furthermore, we investigated how the forest soil microbial absolute content and distribution change with various environmental factors. The major conclusions are as follows:(1)A total of 12 soil samples of different vegetation types from Pingding Mountain, Changbai Mountain and Saihanwula Mountain background forests were analyzed for PLFAs, C/N, TOC, and pH, following the field test of their environmental factors such as soil temperature and moisture. The results showed that the total organic carbon (TOC) ranged from 3.15%~16.3%, the carbon to nitrogen ratio (C/N) ranged from 12.1~18.4, the pH value ranged from 3.5~4.8, the moisture content ranged from 13.3%~74.5%and the soil temperature while sampling was 8.0~18.8 °C, respectively. (2)The total PLFAs content represents the biomass of soil microbes, which was in the range of 27.39~237.63 µg·g-1. Soil microbial biomass in Saihanwula Moutain was highest among the three mountains, while the contents of bacteria, fungi and G+-bacteria in Pingding Moutain were significantly higher than the other two mountains. The ratio of G+-bacteria to G--bacteria (G+/G-) in Pingding Mountain (4.19) was also higher than Changbai Mountain (3.14) and Sahanwula Mountain (2.39). On the contrary, the ratio of fungi to bacteria (F/B) was totally different from G+/G-with Saihanwula Moutain (1.05) being the highest, followed by Changbai Mountain (0.69) and Pingding Mountain (0.55). (3)We examined the correlation between different microbial communities and environmental factors using SPSS software. The results showed that the content of bacteria in forest soil significantly correlated with latitude and C/N (P<0.01);fungi were negatively correlated with C/N (P<0.05) generally. Further correlated analysis between two main PLFAs representing fungi (C18:1ω9、C18:2ω6,9) with C/N demonstrated that C18:1ω9 was significantly correlated with C/N (P<0.01), whereas C18:2ω6,9 was not significantly correlated with C/N. Therefore, the single PLFA as biomarker is probably more sensitive to the change of C/N. (4)The principal components analysis indicated that the soil biodiversity was determined by the vegetation types (P<0.01), and it was influenced by soil C/N and soil moisture content as well (P<0.05).关键词
磷脂脂肪酸/森林土壤/微生物群落结构/平顶山/长白山/赛罕乌拉Key words
phospholipid fatty acid/forest soil/microbial community structure/Pingding Mountain/Changbai Mountain/Sahanwula Mountain分类
农业科技引用本文复制引用
姜懿珊,肖静,罗春玲,李军,张干..平顶山、长白山、赛罕乌拉森林土壤微生物群落结构分析[J].生态环境学报,2014,(8):1272-1279,8.基金项目
国家杰出青年基金项目 （）
