生态环境学报Issue(8)：1320-1326,7.
液相色谱结合串联质谱方法研究中国沿海贝类中脂溶性藻毒素的种类结构和分布规律
The Lipophilic Phycotoxins Profile and Distribution in Bivalve Shellfish of Chinese Coasts by High Performance Liquid Chromatography Coupled with Mass Spectrometry
摘要
Abstract
A number of marine microalgae are known to produce toxins that can accumulate in shellfish, leading to food poisons and potentially fatal reactions of humans and other mammals consuming the contaminated shellfish. Lipophilic phycotoxins are dissolvable in organic solvents, and easily accumulated in animal adipose tissue. There are six common types lipophilic phycotoxins with variety of structures for each. This paper reported the profiles and distributions of lipophilic phycotoxins in 34 bivalve species from 55 stations along the coasts of China. Multiple types of lipophilic phycotoxins were detected in the bivalves along the coasts of China through the HPLC-MS/MS method, including Okadaic acid (OA), Pectenotoxin (PTXs), Azaspiracid (AZAs), Yessotoxin (YTXs)、Gymnodimine(GYM) and Spiroloides(SPX)etc. GYM was mostly observed in the coastal water of Guangdong, Guangxi and Hainan in South China Sea;YTXs, mainly present in Patinopecten yessoensis and Chlamys farreri, were detected only in Yellow Sea; while PTXs were mostly from the coast of Bohai. We observed three structures of PTXs, among which, the one had highest positive rate of 44% and maximum tissue concentration of 53.2 µg·kg-1 were PTX-2Sa; In terms of positive rate and tissue concentration, GYM (35.3%,39.1 µg·kg-1) followed PTX-2Sa. Similarly, three structures of YTXs were detected. And YTXs were present in the tissues of Patinopecten yessoensis in northern Yellow Sea throughout the year with a peak concentration in September. The geographic distributions of phycotoxins could be depended on the presence of toxic algae, while it was still unclear about the biological source of these three common lipophilic phycotoxins detected in the shellfish in China. Although the level of lipophilic phycotoxins in this study were lower than the regulated limitation for food safety, the work here reported the wide presence of variety of phycotoxins in the shellfish along the coasts of China, posing potential health risks for shellfish consumers in these regions. We recommend the great effort should be paid on monitoring of algae toxins and toxin-producing algae to ensure the risk of bivalve consumption is managed.关键词
脂溶性藻毒素/液相色谱-串联质谱/扇贝毒素PTXs/虾夷扇贝毒素YTXs/环亚胺毒素GYMKey words
lipophilic phycotoxins/HPLC-MS/MS/pectenotoxin/yessotoxin/gymnodimine分类
资源环境引用本文复制引用
刘仁沿,梁玉波,刘磊,樊东红,许道艳,孙茜..液相色谱结合串联质谱方法研究中国沿海贝类中脂溶性藻毒素的种类结构和分布规律[J].生态环境学报,2014,(8):1320-1326,7.基金项目
国家自然科学基金项目（41176086；41276099）；海洋公益性行业科研专项（201305010）；国家重点基础研究发展规划（973计划）(2010CB428706) （2010CB428706）
