生态环境学报Issue(1)：113-120,8.DOI:10.16258/j.cnki.1674-5906.2015.01.017
一株贫营养异养硝化-好氧反硝化菌的筛选及脱氮特性
Screening and Nitrogen Removal Characteristics of A heterotrophic Nitrification-aerobic Denitrification Strain
摘要
Abstract
To investigate the feasibility that the strain can used to treat micro-polluted water, the research is mainly about the identification and the denitrification characteristics of the aerobic-denitrification strain that was isolated from sediment in water reservoir and the effects of nitrogen removal under different environmental factors. The optimal conditions of the strain can be determined. Through the method of cultivation and acclimatization to make the strain adapt to the oligotrophic condition and make effects on nitrogen removal, which all prove a theoretical basis for the practical application of onsite biological treatment in the future. A high efficient heterotrophic nitrification-denitrification aerobic bacteria (A14) was isolated from sediment in water reservoir. Based on its microscopic observation, biochemical/morphological characteristics, its 16S rRNA sequence homologic analysis and Biolog GenⅢ identification, this strain was a gram-negative bacillus and was identified as Acinetobacter pittii. The efficient nitrogen removal in 36 hours can be 78.89%when nitrogen as the nitrogen source and the strain’s partial napA functional gene was cloned. When ammonia as the nitrogen source, TN removal rate can be 80.42%, ammonia removal rate can be 95.25%, TOC removal rate can be 98.3%. The highest nitrite removal rate reaches 86.62% when carbon resource is NaAc, temperature=30 ℃, C/N=12, pH=7, inoculating ratio is 10%. In addition, the nitrite removal rate can reach 40.18%under 10 ℃. When the strain is cultivated and acclimated in reservoir raw water, TN removal can be 50.95%, the nitrogen removal can be 80.25%. It is therefore demonstrated that the strain A14 can be used to treat micro polluted water.关键词
异养硝化/好氧反硝化/微污染水源水/功能基因Key words
heterotrophic nitrification/aerobic denitrification/micro polluted water/functional genes分类
资源环境引用本文复制引用
黄廷林,张丽娜,张海涵,苏俊峰,郭琳,赵金亚,张凯..一株贫营养异养硝化-好氧反硝化菌的筛选及脱氮特性[J].生态环境学报,2015,(1):113-120,8.基金项目
国家科技支撑计划项目（2012BAC04B02）；国家自然科学基金项目 （）
