生态环境学报Issue(1)：133-138,6.DOI:10.16258/j.cnki.1674-5906.2015.01.020
湖南石门雄黄矿区矿样中的砷氧化细菌的分离及鉴定
Isolation and Identification of Arsenic-oxidizing Bacteria from Shimen Realgar Mine, Hunan
摘要
Abstract
With the rapid industrialization, especially the arsenic containing gold ore mining, arsenic contamination has become a universal concerned eco-environmental problem. How to make better use of a rapid and green approach in biology to disposal of arsenic wastewater could be of great value in relieving arsenic contamination brought by industrial development, improving residents' quality of life and improving human settlements. In the present study, three samples were used as the research objects, which were collected from the water of tailings pond (SY), mine drainage from the mine tunnel 400 m below the surface (XY) and the sediment of tailings pond (NY) in the Shimen Realgar Mine, respectively. Three kinds of As(Ⅲ)-oxidizing microfloras were obtained from these three samples by enrichment culture with arsenic containing medium and subculture after several generation. The As(Ⅲ) tolerance ability was determined in the medium with l~5 g·L-1 of arsenic. Results showed that the cell density of these microfloras reached 109 cells·mL-1 within 2~3 d under 5 g·L-1 of arsenic. Results also showed that the solution containing 1 g·L-1 of sodium arsenite could be completely oxidized by SY, XY and NY within 25 h, 20 h and 35 h respectively, with the decrease of total arsenic mass concentration by 66.7%. 11 As(Ⅲ)-oxidizing bacteria were obtained from those microfloras by repeated plate streaking and qualitative AgNO3 screening method. The 16S rDNA of these strains were examined by sequence alignment of the BLASTN in NCBI. Results showed strains SMY24, SMY33, SMY22, SMY32, SMY21 and SMY31 are Pseudomonas, SMY104 is Acinetobacter. of all known sequences, the maximum rate of sequence similarity of SMY17, SMY25, SMY9 and SMY1 is only 91%, while the minimum is 76%, indicating they are pure culture bacteria that never been reported. By measuring the As(Ⅲ)-oxidizing ability of each strain, results showed SMY104 and SMY21 could completely remove As(Ⅲ) within 20 h. Preliminary tests for the As(Ⅲ) oxidase gene showed that most of these strains had the arsenic oxidase gene (AoxB).关键词
湖南石门雄黄矿/三价砷/五价砷/砷氧化菌/三价砷氧化酶基因Key words
Shimen realgar mine in Hunan/As(Ⅲ)/As(Ⅴ)/arsenic-oxidizing bacteria/arsenic oxidase gene分类
资源环境引用本文复制引用
杨宇,白飞,杨莉,许庆,邱冠周..湖南石门雄黄矿区矿样中的砷氧化细菌的分离及鉴定[J].生态环境学报,2015,(1):133-138,6.基金项目
国家重点基础研究发展计划（“973”计划）项目（2010CB630901）；教育部新世纪人才项目 （）
