武警医学Issue(9)：912-915,4.
CML28与 WT1在急性白血病细胞中的表达及相关性
Expression level and correlation between CML28 and WT1 in acute leukemia patients ’ bone marrow cells
白雪玲 1庄红丽1
作者信息
- 1. 郑州 450052，武警河南总队医院肿瘤血液科
- 折叠
摘要
Abstract
Objective To study the expression level and correlation between CML 28 and WT1 in acute leukemia ( AL) pa-tients’ bone marrow( BM) primary cells.Methods Bone marrow mononuclear cells ( MMNC) were collected from 29 AL patients who were initially treated and admitted to the Tongji Hospital affiliated to Huazhong University of Science&Technology from January to June in 2009.Total mRNA was extracted from MMNC of AL patients using Trizol reagents , and the first cDNA was synthesized using reverse transcription kits .Their mRNA expression levels were detected in AL patients by RT-PCR and real time PCR and their mRNA expression levels were quantitated by relevant quantitative formula 2 -ΔΔCT .Then the correlation coefficient was calculated using SPSS 17.0 statistical software.Results There was a high expression level of CML28 and WT1 in the MMNC of AL patients at the same time, with significant difference between their mRNA expression leuels (r=0.462,P=0.008).Conclusions CML28 and WT1 are highly expressed in a variety of acute leukemia , and they have an obviously positive correlation .关键词
CML28/WT1/急性白血病/相关性Key words
CML28/WT1/acute leukemia/correlation分类
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白雪玲,庄红丽..CML28与 WT1在急性白血病细胞中的表达及相关性[J].武警医学,2014,(9):912-915,4.
