武警医学Issue(1)：79-82,4.
钙调蛋白和钙／钙调蛋白依赖性蛋白激酶Ⅱ在大鼠海马发育中的变化规律
Expression of Calmodulin and Ca2+/calmodulin kinaseⅡ in rat hippocampus during de-velopment stage
摘要
Abstract
Objective To study the changes in expressions of CaM、CaMKⅡmRNA and protein in the hippocampus of rat dur-ing postnatal development .Methods Real-time PCR and Western blot were used to detect the different expressions of mRNA and pro-tein levels of CaM,CaMKⅡ,respectively, in postnatal day 1, day 7, day 14, day 21, day 30 and day 50.Results The expression of CaM and CaMKⅡwere weaker in the first day after birth, but increased rapidly at postnatal days 14-30 (P<0.05 or P<0.01).The expressions continued to a higher level at postnatal day 50 ( the relative values of CaMmRNA and CaMKⅡmRNA were 2.242 ±0.154 and 2.418 ±0.329, respectively;the relative values of CaM protein and CaMKⅡprotein were 0.782 ±0.061 and 2.194 ±0.256, re-spectively)(P<0.05 or P<0.01), but had low expression trend compared with postnatal day 21.Conclusions There is certain time sequence in the expressions of CaM/CaMKⅡmRNA and protein in the hippocampus of rat during postnatal development .This pat-tern may be closely related to the change of synaptic development and brain maturity .It is also closely related to the formation of learn-ing and memory .关键词
钙调蛋白/钙/钙调蛋白依赖性激酶Ⅱ/海马/认知Key words
calmodulin/Ca2+/calmodulin kinaseⅡ/hippocampus/cognition分类
医药卫生引用本文复制引用
王少博,牟心红,孟斌,付正英,张引国..钙调蛋白和钙／钙调蛋白依赖性蛋白激酶Ⅱ在大鼠海马发育中的变化规律[J].武警医学,2015,(1):79-82,4.基金项目
国家自然科学基金（81271224）；武警后勤学院创新团队基金 （）
