武警医学Issue(6)：603-605,608,4.
EGB761对 VH－64尤文肉瘤细胞的促凋亡作用
Effection of EGB 761 to apoptosis of Ewing’ s sarcoma VH-64 cell in vitro
李战宁 1孟宏涛 1闫鲲 1崔海斌 2郭义 2刘晶1
作者信息
- 1. 710054 西安，武警陕西总队医院医务处
- 2. 710054西安，武警陕西总队后勤部卫生处
- 折叠
摘要
Abstract
Objective To explore the contribution of EGB761 to the proliferation and apoptosis of Ewing’ s sarcoma VH-64 cell in vitro.Methods CCK-8 assay was used to measure 50,100 and 200 mg/L which its effects on the proliferation of Ewing’s sar-coma VH-64 cell cultured with different concentrations of allicin for 12 h, 24 h, 48 h, AnnexinV-FITC and PI assay by flow cytometer ( FCM) were applied to detect the apoptosis induced by EGB761.The changes of cell cycle were detected by FCM.Results EGB761 suppressed the proliferation of Ewing’ s sarcoma VH-64 cell in a concentration-and time-dependent manner EGB761 induced cellular apoptosis of the Ewing ’ s sarcoma VH-64 cell in a concentration-dependent manner.With EGB761 100, 200 mg/L intervention Ewing’s sarcoma VH-64 cell, flow cytometer (FCM) test showed that the cell apoptosis rate were 6.85%±0.36% and 14.36%± 0.23%,which were significant higher than that of control group (P<0.01).Conclusions EGB761 significantly suppresses the growth of Ewing’ s sarcoma VH-64 cell, induces the apoptosis of Ewing’ s sarcoma VH-64 cell.Therefore resveratrol may be considered as a possible treatment medicine for Ewing’ s sarcoma.关键词
EGB761/VH-64/尤文肉瘤/凋亡Key words
EGB761/VH-64 cell/Ewing’ s sarcoma/apoptosis分类
医药卫生引用本文复制引用
李战宁,孟宏涛,闫鲲,崔海斌,郭义,刘晶..EGB761对 VH－64尤文肉瘤细胞的促凋亡作用[J].武警医学,2015,(6):603-605,608,4.
