外科理论与实践Issue(2)：126-130,5.DOI:10.16139/j.1007-9610.2015.02.009
胆固醇结石病人胆汁免疫球蛋白含量增高及其对胆固醇-磷脂泡的影响
Effect of increased billiary immunoglobulins on formation of cholesterol-phospholipid vesicle in patients with cholesterol gallstone
汪四七 1蔡端 1查锡良 2张群华 1张延龄1
作者信息
- 1. 复旦大学附属华山医院普外科，上海 200040
- 2. 复旦大学上海医学院生化系，上海 200032
- 折叠
摘要
Abstract
Objective To study the contents of IgA, IgG or IgM from both bile and serum in the patients with cholesterol gallstone and control groups, and the effect of immunoglobulin on cholesterol-phospholipid vesicles. Methods IgA, IgG and IgM were purified by affinity chromatography using ConA-sepharose, and (twice) Sephadex G-200 gel filtration from bile and serum. The content of IgA, IgG or IgM was determined by immunodiffusion assay. The alteration of cholesterol-phospholipid vesicles was observed under electron microscopy. Results The content of IgG and IgM from gallbladder bile in cholesterol gallstone group was much higher, compared with that of control groups. The content of IgA, IgG and IgM from common bile duct bile in cholesterol gallstone group also increased. However, the content of IgA and IgM from common bile duct bile in pigment gallstone group was same as control groups. No differences were found between the groups in the content of immunoglobulins in serum except for lower content of IgA in group of cholesterol gallstone and pigment gallstone. Either IgG or IgM added in model bile for 7 day enlarged obviously the diameter of cholesterol-phospholipid monolayer vesicles and increased the numbers of cholesterol-phospholipid multilayer vesicles. IgA enlarged slightly the diameter of cholesterol-phospholipid monolayer vesicles, but did not affect the number of cholesterol-phospholipid multilayer vesicles. Conclusions The increased content of IgA, IgG and IgM may facilitate the cholesterol gallstone formation through altering the cholesterol-phospholipid vesicles.关键词
胆囊胆固醇结石/胆汁/免疫球蛋白/胆固醇-磷脂泡Key words
Cholesterol stone/Biliary/Immunoglobulin/Cholesterol-phospholipid vesicle分类
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汪四七,蔡端,查锡良,张群华,张延龄..胆固醇结石病人胆汁免疫球蛋白含量增高及其对胆固醇-磷脂泡的影响[J].外科理论与实践,2015,(2):126-130,5.
