物理化学学报Issue(3)：483-488,6.DOI:10.3866/PKU.WHXB201501151
基于DNA电化学发光传感器研究金纳米颗粒对量子点的电化学发光影响
Effects of Gold Nanoparticles on Quantum Dot Electrochemiluminescence Obtained Using a DNA Electrochemiluminescence Sensor
摘要
Abstract
Gold nanoparticles (AuNPs) have a high extinction coefficient and a strong surface plasmon resonance, the latter of which is influenced by the size of AuNPs and the surrounding environment. In this article, a DNA electrochemiluminescence (ECL) sensor was fabricated based on the distance-dependence of semiconductor nanocrystals' ECL signal to AuNPs. AuNPs were first deposited on the surface of glassy carbon electrode (GCE) by cyclic voltammetry (CV). The mercaptopropionic acid-capped CdS quantum dots (QDs) used in this study can covalently bind with amino-terminated double-stranded DNA (dsDNA), via the―CO―NH bond to obtain a QDs-dsDNA compound. The QDs-dsDNA compounds were assembled on the surface of AuNPs via an Au―S bond, using the other distal of dsDNA that is labeled with thiol, to create the CdS QDs-DNA/AuNPs/GCE ECL sensor. Experimental conditions, such as the QDs-dsDNA density on the surface of electrode and the deposition method of AuNPs, were then optimized. The surface properties of different modified electrodes were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and electrochemical impedance spectroscopy (EIS). The effect of AuNPs on the ECL intensity of CdS QDs was investigated by control ing the DNA which lies between the AuNPs and the CdS QDs. The ECL signal was affected significantly by the length and type of DNA strands. The sensor was used to detect DNA damage from environmental pol utants and exhibited a highly sensitive response.关键词
量子点/电化学发光/金纳米颗粒/DNA/全氟辛酸Key words
Quantum dot/Electrochemiluminescence/Au nanoparticle/DNA/Perfluorooctanoic acid分类
化学化工引用本文复制引用
鲁理平,李娇,武静,康天放,程水源..基于DNA电化学发光传感器研究金纳米颗粒对量子点的电化学发光影响[J].物理化学学报,2015,(3):483-488,6.基金项目
The project was supported by the National Natural Science Foundation of China (21005005,21375005,21475006) and Beijing Nova Program, China (2010B009).国家自然科学基金(21005005,21375005,21475006)和北京市科技新星项目(2010B009)资助 （21005005,21375005,21475006）
