西安交通大学学报（医学版）Issue(2)：169-174,6.DOI:10.7652/jdyxb201502005
miR-185∗对癫痫海马神经元中 BDNF-TrkB 通路表达的影响
Effect of miR-185 * on BDNF/TrkB signaling pathway expression in epileptic neurons
摘要
Abstract
ABSTRACT:Objective To study the BDNF/TrkB signaling pathway in the epilepsy model of hippocampal neurons and the regulatory effect of on it.Methods The primary hippocampal neurons cultured in vitro for 7 days were randomly divided into seven groups:control group,epilepsy group,control+BDNF group,epilepsy+BDNF group,control + miR-185 ∗ group,epilepsy + miR-185 ∗ group,and epilepsy + miR-185 ∗ + BDNF group.We constructed miR-185 ∗ lentivirus vector and observed the changes of BDNF/TrkB pathway expression after transfaction of miR-185 ∗ by immunohistochemistry,patch clamp technique and Western blot technique.Results Compared with the control+BDNF group,the phosphorylated TrkB (pTrkB)/TrkB value was significantly lower in epilepsy+BDNF group (P < 0.05 )and control group (P < 0.001 ).Compared with the epilepsy group,the phosphorylated TrkB (pTrkB)/TrkB value was significantly higher in epilepsy+BDNF group (P <0.05).Compared with the epilepsy+miR-185 ∗ +BDNF group,the phosphorylated TrkB (pTrkB)/TrkB value was significantly lower in epilepsy + BDNF group and epilepsy + miR-185 ∗ group (P < 0.001 ).BDNF could promote the signaling conduction and miR-185 ∗ could remove the inhibition of BDNF/TrkB signaling.Conclusion BDNF can activate the BDNF/TrkB signaling pathway and transfection with miR-185 ∗ can relieve the inhibition of BDNF/TrkB signaling pathway of epileptic state by up-regulating the expression of TrkB.关键词
miR-185∗/BDNF/TrkB/海马神经元/癫痫/蛋白印迹Key words
miR-185 ∗/BDNF/TrkB/hippocampal neuron/epilepsy/Western blot分类
医药卫生引用本文复制引用
蔡浩,李江丽,谢伟,常伟,宋毅军..miR-185∗对癫痫海马神经元中 BDNF-TrkB 通路表达的影响[J].西安交通大学学报（医学版）,2015,(2):169-174,6.基金项目
国家自然科学基金(No.91132722,81071044) （No.91132722,81071044）
