西安交通大学学报（医学版）Issue(2)：219-224,6.DOI:10.7652/jdyxb201502015
Ad36感染对维吾尔族肥胖患者progranulin 表达的调节作用
The regulation of adenovirus type 36 infection and progranulin expression in Uygur obese patients
摘要
Abstract
ABSTRACT:Objective To investigate the regulation of adenovirus type 36 infection on precursor protein particles progranulin expression in Uygur obese patients.Methods Based on the diagnostic criteria of obesity,the samples were divided into obese group and non-obese group.Serum neutralization test was used to detect the antibody of Ad36.The progranulin mRNA expressions in abdominal omental and subcutaneous adipose tissues were detected by real-time quantitative PCR.ELISA method was used to determine serum progranulin protein levels. CD68 protein expression of macrophage was detected by immunohistochemistry. Results ① In the Uygur population of our study,compared with that in the non-obese group (38/1 1 7,32.5%),the number of obese patients (54/1 1 5,47.0%)infected with Ad36 was significantly higher than that in non-obese group (P <0.05 ).② Serum progranulin was significantly increased in the Ad36-infected obese group (408.45±1 56.92)than in non-obese group (326.1 1±1 58.60)(P <0.05).The mRNA expression of progranulin did not differ between the two groups.③ The macrophage infiltration was significantly higher in the Ad36-infected obese group (14 730.1 6 ± 2 227.39 )than in non-obese group (10 786.50 ± 2 772.80 )(P < 0.05 ).Conclusion Ad36 infection may be associated with the occurrence of obesity in Uygur population,and adenovirus type 36 infection may regulate the expression of serum progranulin at the protein level.关键词
腺病毒 36 型/肥胖/颗粒蛋白前体(progranulin)/实时荧光定量-聚合酶链反应(real time quantitative-PCR)/巨噬细胞/维吾尔族Key words
Ad36/obesity/progranulin/real time PCR/macrophages/Uygur分类
医药卫生引用本文复制引用
常曦,焦谊,陆剑飞,努尔比耶·努尔麦麦提,关亚群..Ad36感染对维吾尔族肥胖患者progranulin 表达的调节作用[J].西安交通大学学报（医学版）,2015,(2):219-224,6.基金项目
国家自然科学基金(No.30960363) （No.30960363）
博士(后)科研启动基金(No.2012-7) （后）
