西安交通大学学报（医学版）Issue(2)：271-274,284,5.DOI:10.7652/jdyxb201502024
大蒜素对脑胶质瘤细胞 U87细胞侵袭能力的影响及其机制
The effect and mechanism of allicin on the migration and invasion properties of human osteosarcoma U87 cells
蔡青 1秦怀洲 1陈昆仑2
作者信息
- 1. 第四军医大学唐都医院神经外科，陕西西安 710038
- 2. 西安交通大学医学部第二附属医院普外科，陕西西安 710004
- 折叠
摘要
Abstract
ABSTRACT:Objective To investigate the anti-metastatic effect of allicin on glioma cell line U87 and related mechanisms.Methods In this study,we employed MTT assay to test the anti-proliferative effect of allicin. Transwell assay was used to test the anti-metastatic ability of allicin.Real-time PCR and Western blotting were employed to test the effect of allicin on the expressions of matrix metalloproteinase-2 (MMP-2 ) and matrix metalloproteinase-9 (MMP-9).Western blotting was employed to test the phosphorylated level of p38.Results Allicin could significantly inhibit the proliferation and invasion of U87 cells (concentration>8 μg/mL,P <0.05). Meanwhile allicin (concentration<8μg/mL)could inhibit the invasion of U87 cells.After treatment with allicin for 24 hours,the expressions of MMP-2 and MMP-9 were decreased significantly (P < 0.05 ).Moreover,allicin treatment decreased the phosphorylated level of p38 obviously (P < 0.05 ).Conclusion Allicin inhibits the invasion and migration of glioma cell line U87 by reducing the expressions of MMP-2 and MMP-9 via suppressing the activity of p38 signal pathway,suggesting that allicin is a potential therapeutic agent for glioma.关键词
脑胶质瘤/大蒜素/侵袭/MMPs/p38/U87 细胞Key words
glioma/allicin/invasion/matrix metalloproteinases (MMPs)/p38/U87 cells分类
医药卫生引用本文复制引用
蔡青,秦怀洲,陈昆仑..大蒜素对脑胶质瘤细胞 U87细胞侵袭能力的影响及其机制[J].西安交通大学学报（医学版）,2015,(2):271-274,284,5.
