西安交通大学学报（医学版）Issue(3)：299-303,309,6.DOI:10.7652/jdyxb201503003
帕金森病大鼠伏隔核神经元电活动变化及5-HT7受体激活的作用
Changes in the firing activities of the nucleus accumbens and effect of 5-HT7 receptor activation in a rat model of Parkinson’s disease
摘要
Abstract
Objective To investigate changes in the firing activities of nucleus accumbens (NAc)neurons and their response to 5-HT7 receptor stimulation in a rat model of Parkinson’s disease (PD).Methods The firing activities and response of NAc neurons to 5-HT7 receptor agonist in PD rats were recorded by in vivo electroneurophysiology and neuropharmacology and then were compared with those in the sham group.Results The mean firing rate of NAc neurons was (5.46 ±0.88)Hz in the sham rats and (3.77 ±0.48)Hz in the PD rats. The firing rate of NAc neurons increased significantly compared with that in the sham rats (P <0.05).In PD rats, 65% of NAc neurons fired in bursts and 35% fired irregularly.However,in the sham rats,57.5% of NAc neurons fired in bursts and 42.5% fired irregularly.There was no significant difference in the firing pattern of NAc neurons between the PD and sham rats (P >0.05 ).Systemic administration of 5-HT7 receptor agonist AS1 9 increased the firing rate of NAc neurons in the sham and PD rats.This excitation was significant at a high dose of 1 60 μg/kg for NAc neurons in the sham rats (P <0.05).However,the excitation produced by AS1 9 was significant at a high dose of 80 μg/kg in PD rats (P <0.05).The cumulative dose-produced excitation in the PD rats was lower than that in the sham rats.The effects induced by AS1 9 were reversed by the 5-HT7 receptor antagonist SB269970 in both groups.Conclusion The reinforced firing activity of NAc neurons might be mediated by 5-HT7 receptor in the neurons of PD rats.关键词
伏隔核/帕金森病/5-HT7 受体/电生理学/大鼠Key words
nucleus accumbens/Parkinson’s disease (PD)/5-HT7/electrophysiology/rat分类
医药卫生引用本文复制引用
张欢,吴仲恒,李立博,乔鸿飞,袁海峰,杜成学,谭会会,张巧俊..帕金森病大鼠伏隔核神经元电活动变化及5-HT7受体激活的作用[J].西安交通大学学报（医学版）,2015,(3):299-303,309,6.基金项目
国家自然科学基金(No.81171215)及西安交通大学医学部第二附属医院人才培养专项科研基金资助[No.RC(GG)201104] Supported by the National Natural Science Foundation of China (No.81171215)and the Scientific Research Foundation for Personnel Training of the Second Affiliated Hospital of Xi’an Jiaotong University [No.RC(GG)201104) （No.81171215）
