陕西科技大学学报（自然科学版）Issue(6)：129-133,5.
D-阿拉伯糖醇高产酵母菌株的快速筛选方法
A rapid screening method of yeast strains for hight yield production of D-arabitol
摘要
Abstract
A high throughput screening method that using high sugar medium for preliminary screening and paper chromatography in combination with high performance liquid chroma‐tography for further screening was established to selectively breed the high‐yield D‐arabitol strain by low‐energy N+ ion implantation .The expand system ,sample volume and chromoge‐nic method of paper chromatography were optimized .The results indicate that optimal condi‐tions are as follows :eluent∶ n‐butanol∶pyridine∶water=14∶3∶3 ,sample volume:1 .5μL , chromogenic agents 50% acetone solution of silver nitrate and 50% sodium hydroxide solu‐tion in ethanol .High‐yield D‐arabitol producing strains were obtained based on the size of significant colored spots ,then high performance liquid chromatography was used to verify the result .The results show that this method can be used to screen quickly D‐arabitol high‐yield strains from large quantities of mutated strains .The high‐yield strain has good genetic stabil‐ity and its production was 88 .23 g/L .关键词
低能N+离子/D-阿拉伯糖醇/高糖培养基/纸色谱/高效液相色谱Key words
low-energy N+ ion/D-arabitol/high sugar medium/paper chromatography/high performance liquid chromatography分类
生物工程引用本文复制引用
钱卫东,宁肖肖,王兰,蔡长龙,毛培宏,李梦媛..D-阿拉伯糖醇高产酵母菌株的快速筛选方法[J].陕西科技大学学报（自然科学版）,2014,(6):129-133,5.基金项目
国家自然科学基金项目（31100040）；陕西省教育厅自然科学专项科研计划项目（2013JK0727）；西安市未央区科技计划项目 （）
