西北师范大学学报（自然科学版）Issue(3)：79-87,93,10.
中药复方液对麻黄素损伤小鼠卵巢的保护作用
Protection of Chinese medicine compound on ovarian histological structure in filial mice of ephedrine injury
摘要
Abstract
To research the effects of Chinese medicine compound on ovary in filial mice of ephedrine injury , the 60 filial mice of pregnancy are randomly divided into control group , ephedrine group and Chinese medicine group . The control group is injected with saline , the ephedrine group and Chinese medicine group are injected with 0.2 mL 6.0 g·L -1 the ephedrine , and Chinese medicine group are filled with 0.3 mL of Chinese medicine compound after injecting ephedrine 1 h . The concentration changes of EPI and E2 in plasma is detected by ELISA method , the changes of the SOD activity and MDA content are analyzed by colorimetry , the ovary weight are measured and the change of ovarian histological structure are observed by microtechnique , counted the numbers changes of secondary follicle and atretic follicle , measured the diameter change of secondary follicle . T he expression changes of Bax protein in the ovary are measured by immunohistochemistry method . Results indicate that the E2 concentration in plasma of filial mice decreases and the EPI concentration increasess in the ephedrine group . The activity of SOD is lower , MDA content significantly increases , the ovary weight is lower of ephedrine group , the numbers of secondary follicle and atretic follicle increas and the diameters of secondary follicle decrease in the ephedrine group compared with control group , there are pyknotic ovarian tissues , including degradation granulose cells , karyopyknotic granulose lutein cells in the ephedrine group . But the EPI concentration in plasma of filial mice decreases and the E2 concentration increases in the Chinese medicine group , the activity of SOD significantly increases , MDA content is lower , the structure of ovarian is clear , the numbers of secondary follicle decreases , the overy structure of cumulus is clear , granulosa cells closely arrange , disintegration reduce , the expression of Bax protein in the ovary significantly decreases compared with ephedrine group . The results show that Chinese medicine compound can protect ovarian ephedrine injuried , that may be correlated with the activity of SOD and the expression of Bax protein .关键词
受孕小鼠/中药复方液/麻黄素/卵巢组织结构Key words
pregnant mice/Chinese medicine compound/ephedrine/ovarian histological structure分类
医药卫生引用本文复制引用
俞诗源,刘小媛,吴转子,王敏,曹力凡..中药复方液对麻黄素损伤小鼠卵巢的保护作用[J].西北师范大学学报（自然科学版）,2014,(3):79-87,93,10.基金项目
甘肃省自然科学基金资助项目（1107RJZA141）；兰州市社会发展研究项目 （）
