西北师范大学学报（自然科学版）Issue(2)：66-72,84,8.
维生素C和维生素E的联合抗氧化活性研究
Antioxidant activity studies of vitamin C and vitamin E
摘要
Abstract
Vitamin C and Vitamin E have many physiological activities , and both are antioxidant . However , their combinative actions in biochemistry system are rarely reported . In this paper , the methods of K3 Fe (CN )6 ,methyl violet , pyrogallol autoxidation and DMPD・ + are used to measure the reducing activity , the scavenging ability on hydroxyl radical , superoxide anion and DMPD・ + of vitamin C and vitamin E treatment alone or combination . Moreover , the methods of SDS‐PAGE and SCGE are used to detect the protein oxidative degradation and cell DNA damage . The results indicate that vitamin C and vitamin E exhibits significant antioxidant activities in different antioxidant systems , and the effect shows a concentration‐dependent manner . Vitamin C exhibit stronger abilities of reducing and scavenging free radical than Vitamin E . The combination effect of two Vitamins shows stronger action than anyone alone in most systems , which presents synergistic interaction . In addition , both Vitamin C and Vitamin E play an important role in protecting protein from oxidative damage caused by AAPH , the effect become obvious when two compounds are combined . But their combinative protect actions have not been observed in cell DNA oxidative damage .关键词
维生素 C/维生素 E/自由基/抗氧化活性/联合作用Key words
Vitamin C/Vitamin E/free radical/antioxidant activity/combinative action分类
生物科学引用本文复制引用
刘国安,李杰林,李双越,叶金凤,丁兰..维生素C和维生素E的联合抗氧化活性研究[J].西北师范大学学报（自然科学版）,2015,(2):66-72,84,8.基金项目
国家自然科学基金资助项目（30960464）；教育部留学回国人员科研启动基金资助项目 （）
