西北师范大学学报（自然科学版）Issue(2)：73-77,5.
前列腺癌细胞乳鼠移植瘤动物模型的建立
Establishment of the prostate cancer xenograft model in neonatal mice
摘要
Abstract
To establishment prostate cancer cell (PC3) xenograft model in neonatal mice , PC3 cell strain is culture in vitro and perform the soft agar assay to screen the PC 3 cells for their ability wihch can form colonies in soft agar . After passaged by conventional methods , the cells which in logarithmic growth phase are collect , and then make into single‐cell suspension with cell density of 2 × 10 7 mL - 1 . 0.1 mL single‐cell suspension is injected subcutaneously into the cross point of neck and fore limb in 2 d newborn neonatal mice . The growth characteristics of transplanting PC3 in mice are observed and the tumor properties are identified by histopathology and immunohistochemistry in neonatal mice subcutaneous . Results show there is no adverse reactions in neonatal mice after the injection . The mice subcutaneous swelling and solid bean‐like pallet can be felt in 1 ~ 6 d , the tumor become large and the naked eye can be observed significant oncogenesis in 7 ~ 15 d . But there are signs of tumor regression in 16 ~ 21 d . Pathological HE staining indicates that the mass of oncogenesis are tumor tissue and immunohistochemical staining shows the tumor tissue expression of CD133 strong positive .关键词
PC3/软琼脂克隆形成/乳鼠移植瘤/肿瘤组织Key words
PC3/soft agar colony formation/neonatal mice xenograft/tumor tissue分类
医药卫生引用本文复制引用
曾家豫,张虹,袁红霞,牛童,廖世奇..前列腺癌细胞乳鼠移植瘤动物模型的建立[J].西北师范大学学报（自然科学版）,2015,(2):73-77,5.基金项目
国家自然科学基金资助项目 （）
