现代妇产科进展Issue(1)：18-21,4.DOI:10.13283/j.cnki.xdfckjz.2015.01.005
GPR30在宫颈癌细胞生长中的作用及其机制研究
Study on the effect and mechanism of GPR30 in cervical cancer cell growth
李敏 1闫楠 1唐荣荣 1瞿全新 1钮硙硙 1刘佳佳 1王玉亮2
作者信息
- 1. 天津医科大学一中心临床学院，天津 300192
- 2. 天津市卫生部危重病急救医学重点实验室，天津 300384
- 折叠
摘要
Abstract
Objective:To investigate the effect and mechanism of estrogen receptor GPR30 on the growth of cervical cancer cells in vitro. Methods:Cevrical adenocarcinoma cell line Hela and cervical squamous cancer cell line Siha were treated respectively by GPR30 spe-cific agonist G1 and antagonist G15 . The expressions of GPR30 and TLR3 mRNA and their pro-tein levels were detected by RT-PCR and Western-blot. The effect on cell growth was measured by MTT. Result:(1)The expression of GPR30 was higher in Hela than that of Siha. The mRNA and protein expressions of both Hela and Siha increased after treated by G1 ( P<0 . 05;P<0 . 05 ) and decreased by G15(P<0. 05,P<0. 05). (2)The mRNA expressions of GPR30 in Hela and Siha were (0. 5327±0. 05373) and (0. 3526±0. 05774). And the protein expressions were (0. 3572±0. 097039) and (0. 5002±0. 09718). G1 can reduce HeLa and SiHa cells TLR3 mRNA and protein expression levels. Compared with the control group,the difference was statis-tically significant after G1 10-6mol/L treatment(P<0. 05). While G15 can increase HeLa and SiHa cells TLR3 mRNA and protein expression levels. Compared with the control group, the difference was statistically significant after G1 10-5mol / L treatment(P<0. 05). Compared with the group treated by PolyI:C,the difference was not statistically significant(P>0. 05). (3)After treated by G1 with a concentration of 10-6 mmol/L and 10-5 mmol/L, the polification rates of Hela and Siha were (16. 68±5. 86)%,(26. 67±3. 25)% and (14. 99±6. 43)%,(22. 72±1. 77)%. Compared with the control group, the difference was statistically significant ( P<0. 05). Treated by G15 of 10-6 mmol/L and 10-5 mmol/L,the inhibition rates were (21. 09±2. 32)%,(22. 99±3. 15)% and (15. 86±6. 49)%,(19. 18±2. 61)%. Compared with the control group,the difference was statistically significant(P<0. 05;P<0. 05;P<0. 05;P<0. 05). Conclusion:GPR30 expressed in cervical cancer cells. The expression levels in cervical adeno-carcinoma were higher than those in cervical squamous cell carcinoma. Regulating GPR30 ex-pressions in vitro could influence the growth of cervical cancer cells. Inhibition of GPR30 ex-pressions could inhibit the growth of cervical cancer cells by up-regulating TLR3 expression. GPR30 may become a new target for cervical cancer treatment.关键词
宫颈癌/G蛋白耦联受体30/Toll样受体3Key words
Cervical cancer/G protein-coupled receptor 30/Toll-like receptor 3分类
医药卫生引用本文复制引用
李敏,闫楠,唐荣荣,瞿全新,钮硙硙,刘佳佳,王玉亮..GPR30在宫颈癌细胞生长中的作用及其机制研究[J].现代妇产科进展,2015,(1):18-21,4.
