现代妇产科进展Issue(2)：98-101,4.DOI:10.13283/j.cnki.xdfckjz.2015.02.008
基质成纤维细胞活化促子宫肌瘤细胞增殖作用的研究
The effect of stromal fibroblasts activation on uterine leiomyoma cells proliferation and its mechanism
瞿晓燕 1罗宁 1郭静 1陈丽 1戴虹 1程忠平1
作者信息
- 1. 同济大学附属杨浦医院妇产科 同济大学医学院妇科微创医学研究所，上海 200090
- 折叠
摘要
Abstract
Objective:To observe the activation of stromal fibroblasts promotes the pro﹣liferation of uterine leiomyoma cells,and to investigate the role of stromal fibroblasts cells in the pathogenesis of uterine leiomyoma. Methods:Fibroblast cells from uterine leiomyoma and myo﹣metrium tissue was sorted by immunobead method. The expression of fibroblasts activation mark﹣er (FAP) was detected by immunofluorescence and Western blot. The activation and prolifera﹣tion effect of uterine leiomyoma stromal fibroblasts cell culture supernatant on myometrium stro﹣mal fibroblasts (Fib)、myometrium cells (SMC) and uterine leiomyoma cells (UFC) were test﹣ed. Using silence the FAP gene expression of leiomyoma stromal fibroblasts by RNA interference technology,and detect the cell proliferation ability and ECM、growth factor expression as well as the changing of signal molecule of cell proliferation pathway. Result:The FAP expression level of fibroblasts from leiomyoma tissue was higher than that from myometrium tissue. The cell cul﹣ture supernatant of uterine leiomyoma stromal fibroblasts can enhance the FAP expression of Fib as well as the cell proliferation ability. Silence the expression of FAP gene can inhibit the ex﹣pression of ECM(collgen I、fbronectin、laminin),TGF﹣β、IGF﹣1 and signal molecule of cell pro﹣liferation pathway ( Akt、 ERK、 c﹣Fos、 MEK). Conclusion: The activation level of leiomyoma stromal fibroblasts is higher than myometrium stromal fibroblasts. The activated fibroblast cells can enhance cell proliferation which prompt that the activation of fibroblast cells may play an important role in the pathogenesis of uterine leiomyoma.关键词
子宫肌瘤/成纤维细胞/肿瘤相关成纤维细胞/成纤维细胞活化蛋白Key words
Uterine leiomyoma/Fibroblast cells/Tumor associate fibroblasts/Fibro﹣blast activation protein分类
医药卫生引用本文复制引用
瞿晓燕,罗宁,郭静,陈丽,戴虹,程忠平..基质成纤维细胞活化促子宫肌瘤细胞增殖作用的研究[J].现代妇产科进展,2015,(2):98-101,4.
