现代妇产科进展Issue(3)：161-166,6.DOI:10.13283/j.cnki.xdfckjz.2015.03.001
GPR30在妊娠期胎盘中的表达及其对滋养细胞侵袭能力影响的研究
The research about the expression of GPR30 protein in human placenta and its effect on human trophoblast cells invasion
摘要
Abstract
Objective:To characterise the expression of G—Protein Coupled Receptor 30 (GPR30) in human placenta,and to determine the role of GPR30 in trophoblast invasion. Meth﹣ods:Placentas from first trimester,normal term and pregnancies complicated with pre—eclampsia were used in the study. The effect of 17β—estradiol ( E2 ) ,GPR30 agonist G1 and GPR30 inhibitor G15 on invasion of first trimester villous explants and human trophoblast cell line HTR8/SVneo were determined using in vitro invasion assays. Immunofluorescence and Western blot were used to investigate the GPR30 protein expression. Results:GPR30 protein expression was significantly lower in term placentas from pregnnacies complicated by pre—eclampsia compared to placentas from first trimester and normal term pregnancies. E2 and G1 treatment significantly increased the invasiveness of trophoblast cells. In contrast, the antagonist G15 significantly decreased tropho—blast invasion. In addition,E2 and G1 treatment increased while G15 decreased GPR30 protein ex—pression. Meanwhile,the results also implied that GPR30 involves in regulating the expression of MMP—9. Conclusion:GPR30 plays a role in trophoblast invasion,the up—regulation of GPR30 pro—tein level could increase trophoblast invasion.关键词
GPR30/滋养细胞/侵袭力Key words
GPR30/Trophoblast cells/Invasion分类
医药卫生引用本文复制引用
冯祥,石书明,漆洪波,张华..GPR30在妊娠期胎盘中的表达及其对滋养细胞侵袭能力影响的研究[J].现代妇产科进展,2015,(3):161-166,6.基金项目
国家自然科学基金(No:81100444,No:81370732) （No:81100444,No:81370732）
重庆市自然科学基金资助项目(CSTC,2011BB5121) （CSTC,2011BB5121）
重庆市卫生局科研基金资助项目(No:2011-2-046) （No:2011-2-046）
