现代妇产科进展2015，Vol.24Issue(5)：330-333,4.DOI:10.13283/j.cnki.xdfckjz.2015.05.003
DAPT抑制妊娠滋养细胞肿瘤Notch信号通路对细胞增殖和EMT影响的研究
A study on the role of notch signaling pathway in gestational trophoblastic tumor.
摘要
Abstract
Objective:To study the significance of Notch signaling pathway in the de-velopment of gestational trophoblastic tumor and the relationship between EMT. Methods:The choriocarcinoma cell lines JAR and JEG-3 were treated with gamma-secretase inhibitor ( DAPT) . Using MTT assay to examine the activity of the two cell lines and evaluate the infulu-ence on the proliferation in JAR and JEG-3 cells of DAPT. qPCR was performed to assess mR-NA expression of Notch1. In addition,EMT related protein E-cadherin was detected by Western blot. Results:DAPT could inhibit JEG-3 cells and JAR cells growth,down-regulate the expres-sion of Notch1 mRNA,and up-regulate the expression of E-cadherin in JAR cells. Conclusions:Notch sigaling pathway and EMT may be associated with the development of gestational tropho-blastic tumor. DAPT may be a potential target of new therapeutic investigation in gestational trophoblastic tumor.关键词
妊娠滋养细胞肿瘤/Notch信号通路/EMT/γ-分泌酶抑制剂Key words
Gestational trophpblastic tumor/Notch sigaling pathway/EMT/Gamma-secretase inhibitor分类
医药卫生引用本文复制引用
周园园,薛艳,田泉,安瑞芳..DAPT抑制妊娠滋养细胞肿瘤Notch信号通路对细胞增殖和EMT影响的研究[J].现代妇产科进展,2015,24(5):330-333,4.基金项目
国家自然科学基金资助项目(No:81172489) （No:81172489）
