现代妇产科进展Issue(7)：516-520,5.DOI:10.13283/j.cnki.xdfckjz.2015.07.013
EGFR、蛋白激酶B在宫颈癌中表达及EGFR胞外区基因突变的研究
The expressions of EGFR and PKB protein in cervical cancer and EGFR extracellular region gene mutation
摘要
Abstract
Objective:To investigate the epidermal growth factor receptor ( EGFR ) , protein kinase B ( Akt) expression and significance in cervical cancer;and to analyze the inci-dence of EGFR extracellular region gene mutation in cervical cancer,which provided gene de-tection methods and theoretical basis for the cervical cancer targeted treatment. Methods:The immunohistochemical was used to detect EGFR,Akt expression in 16 cases of normal control, 40 cases with cervical intraepithelial neoplasm( CIN) and 60 cases with cervical cancer. DNA were extracted from 60 cases of cervical cancer by the routine phenol-chloroform method,which were used for PCR amplification and direct sequence for EGFR extracellular region gene muta-tion . Results:The positive rates of EGFR and Akt were 13 . 33% and 6 . 25% in normal cervical tissues,43. 33% and 35. 00% in CIN lesions,76. 67% and 68. 33% in carcinoma respective-ly . The positive expression rate of EGFR in cervical cancer tissues was correlated with the path-ological type,degree of tumor differentiation and clinical staging (P<0. 05),which was no re-lated to age and lymph node metastasis ( P>0 . 05 ) . The positive expression rate of Akt in cervi-cal cancer tissues was correlated with the degree of tumor differentiation and clinical staging ( P <0. 05) which was no related to age,pathological type and lymph node metastasis (P>0. 05). Only two cases EGFR extracellular domain IV 15 exon mutations ( G→A) were detected in 60 cases (3. 33%) cervical cancer while no EGFR extracellular domain IV 14 exon mutations was detected. In cervical cancer,the positive expression rate of EGFR and Akt was positively corre-lated with positive expression rate of Akt ( P<0 . 05 ) . Conclusion:EGFR and Akt are closely associated with precancerous lesions and cervical cancer,and provide new information regarding the treatment strategies for cervical cancer combination therapy. The mutation rate of EGFR ex-tracellular domain was low,there may be other potential or unknown mutation points in human cervical cancer.关键词
宫颈癌/表皮生长因子受体/蛋白激酶B/基因突变/靶向治疗Key words
Cervical cancer/Epidermal growth factor receptor(EGFR)/Protein ki-nase B(Akt)/Gene mutation/Target therapy分类
医药卫生引用本文复制引用
郑凤霞,傅芬,蔡勇,叶称连..EGFR、蛋白激酶B在宫颈癌中表达及EGFR胞外区基因突变的研究[J].现代妇产科进展,2015,(7):516-520,5.基金项目
江西省科技厅基金项目(No:20133BBG70069) （No:20133BBG70069）
