中国组织工程研究Issue(15)：2338-2344,7.DOI:10.3969/j.issn.2095-4344.2014.15.008
大鼠来源滑膜间充质干细胞的成软骨分化
Chondrogenic differentiation of rat synovial-derived mesenchymal stem cells
摘要
Abstract
BACKGROUND:Compared with other sources of mesenchymal stem cells, synovial-derived mesenchymal stem cells have significant characteristics of chondrogenesis and cloning. Therefore, synovial-derived mesenchymal stem cells are one of the most promising seed cells in cartilage tissue engineering. <br> OBJECTIVE:To isolate and culture synovial-derived mesenchymal stem cells of Sprague-Dawley rats, identify the multipotential differentiation and the potential ability of chondrogenic differentiation in three-dimensional culture condition. <br> METHODS:The synovium tissue was harvested from Sprague-Dawley rats. The synovial-derived mesenchymal stem cells were isolated with typeⅠcol agen enzyme digestion method and cultured in vitro. The passage 3 cells were detected with giemsa staining, the cellcycle, adipogenic and osteogenic differentiation were determined. The passage 3 cells were centrifuged as pel ets and cultured in the chondriogenic medium for 21 days. And the pel ets were examined by toluidine blue staining, typeⅡcol agen immunohistochemical staining and RT-PCR. <br> RESULTS AND CONCLUSION:The mesenchymal stem cells isolated from the synovium tissue of rats have the characteristics of mesenchymal stem cells, and exhibit fibroblast-like morphology after cultured in vitro. The multilineage differentiation potentials were also revealed. After the cellwere cultured in chondrogenic medium for 21 days, chondroid tissue was found, type II col agen and aggrecan could be detected positively by toluidine blue staining, typeⅡcol agen immunohistochemical staining, and expressed by RT-PCR examination. Therefore, synovial mesenchymal stem cells have a chondrogenic differentiation potential.关键词
组织构建/软骨组织工程/干细胞/分化/滑膜间充质干细胞/成骨/成脂/成软骨/生长因子/三维培养/国家自然科学基金Key words
stem cells/mesenchymal stem cells/collagen type II/reverse transcriptase polymerase chain reaction分类
医药卫生引用本文复制引用
邵博,杨萌,林兆全,龚忠诚,刘慧,凌彬,克热木·阿巴斯,尹小朋,胡露露,王冰,宁晓婷..大鼠来源滑膜间充质干细胞的成软骨分化[J].中国组织工程研究,2014,(15):2338-2344,7.基金项目
国家自然科学基金资助项目(31260229)，项目名称PLLA/PDLA三维支架与滑膜间充质干细胞/软骨细胞构建颞下颌关节盘的研究@@@@the National Natural Science Foundation of China, No.31260229 （31260229）
