中国组织工程研究Issue(16)：2519-2525,7.DOI:10.3969/j.issn.2095-4344.2014.16.010
复合壳聚糖微球-丝素基载药α-磷酸三钙骨水泥的细胞相容性及毒性
Cytocompatibility and cytotoxicity of a novel composite bone cement material composed of chitosan microsphere, alpha-tricalcium phosphate and silk fibroin
摘要
Abstract
BACKGROUND:With excelent biocompatibility and osteoconduction, calcium phosphate bone cement has been used in clinic, but the poor mechanical properties and lack of osteoinduction restrict its further use. OBJECTIVE:To investigate the cytocompatibility and cytotoxicity of a novel drug-carrying composite of bone cement composed of chitosan microsphere, α-tricalcium phosphate and silk fibroin. METHODS:MC3T3-E1 cels were cultured in vitroin minimum essential medium alpha medium (α-MEM), which was supplemented with 10% fetal bovine serum, and 1% streptomycin sulfate, extract of the cement material at concentrations of 100% and 50%, and 6.4 mL/L phenol. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to measure cellproliferation and the cytotoxicity was assessed by the activity of lactate dehydrogenase. The MC3T3-E1 cels culturedin vitro were colected and seeded on the composite cement material, and cellmorphology was observed by scanning electron microscope. RESULTS AND CONCLUSION:The extract of composite cement material had no influences on the MC3T3-E1 cellproliferation, showing no obvious cytotoxicity. The scanning electron microscope image showed MC3T3-E1 cels adhered and proliferated wel on the composite cement material composed of chitosan microsphere, α-tricalcium phosphate and silk fibroin, and pseudopodia out of the cels were closely attached to the material surface. In conclusion, the cement composite was proved to have satisfactory cytocompatibility and no obvious cytotoxicity.关键词
生物材料/骨生物材料/丝素基磷酸钙骨水泥/壳聚糖微球/浸提液/MC3T3-E1细胞/细胞增殖/MTT法/乳酸脱氢酶活性/国家自然科学基金Key words
biocompatible materials/calcium phosphates/chitosan/silk分类
医药卫生引用本文复制引用
王金宁,皮斌,王鹏,朱雪松,杨惠林..复合壳聚糖微球-丝素基载药α-磷酸三钙骨水泥的细胞相容性及毒性[J].中国组织工程研究,2014,(16):2519-2525,7.基金项目
国家自然科学基金资助项目(81071451、81171689、81301559)；江苏省自然科学基金资助项目(BK2011264、SBK201341421)；江苏省教育厅研究生创新计划(CXZZ11_0123) （81071451、81171689、81301559）
