中国组织工程研究Issue(16)：2563-2569,7.DOI:10.3969/j.issn.2095-4344.2014.16.017
淫羊藿苷抑制钛颗粒诱导的炎症反应
Icariin inhibits titanium particle-induced inflammatory reaction
摘要
Abstract
BACKGROUND:Studiesin vitro have suggested that icarin can attenuate lipopolysaccharide (LPS)-induced acute pneumonia. Is the anti-inflammatory effect of icarin stil valid in the presence of wear particles? OBJECTIVE:With studiesin vivo andin vitro, to investigate the regulatory effect of icarrin on titanium particle-induced inflammatory reaction. METHODS:(1) Studiesin vivo: Eighty male C57BL/6 mice aged 6-8 weeks were randomly divided into four groups: control group, icarin group, titanium particle group, and titanium particle+icarin group. Mice in the titanium particle group and titanium particle+icarin group received surgical procedure, and sterile and endotoxin-free titanium particles were implanted on the calvaria surfaces to induce inflammatory reaction. Mice in the control group and icarin group received the same surgery, but no wear particles were implanted. Then icarin was given oraly to mice in the titanium particle group and titanium particle+ icarin group with a dose of 200 mg/kg per day for 2 weeks from the day of modeling. Mice in the control group and icarin group were given oraly the same dose of placebo. Two weeks later, tumor necrosis factor-α and interleukin-1β at protein and mRNA levels were respectively detected with enzyme-linked immunohistochemical (ELISA) and quantitative real time reverse transcription PCR (qRT-PCR) analysis. (2) Studiesin vitro: Mouse monocyte/macrophage RAW264.7 cels were cultured with different conditioned media: control group, nuclear factor receptor ligand кB (RANKL); icarin group, RANKL+icarin; titanium particle group, RANKL+titanium particles; titanium particle+icarrin group, RANKL+icarin+titanium particles. Titanium particles stimulated RAW264.7 cels were co-cultured with RANKL and icarin for 72 hours. Tumor necrosis factor-α and interleukin-1β at protein and mRNA levels in the supernatant were detected with ELISA analysis and qRT-PCR, respectively. RESULTS AND CONCLUSION: (1) Resultsin vivo: icarin treatment obviously decreased titanium particle-induced inflammatory cellinfiltration and made the thickness of periosteum thinner, down-regulated tumor necrosis factor-α and interleukin-1β expressions at protein and mRNA levels. (2) Results in vitro: when RAW264.7 cels were stimulated with titanium particles for 72 hours, tumor necrosis factor-α and interleukin-1β expressions at protein and mRNA levels in culture media increased obviously; when icarin was administrated, tumor necrosis factor-α and interleukin-1βexpressions at protein and mRNA levels down-regulated significantly. These results suggest icarin can obviously suppress titanium particle-induced inflammatory reactionin vivo andin vitro.关键词
生物材料/材料相容性/淫羊藿苷/钛颗粒/炎症反应/肿瘤坏死因子α/白细胞介素1β/国家自然科学基金Key words
titanium/systemic inflammatory response syndrome/drugs, chinese herbal/tumor necrosis factor-alpha/interleukin-1beta分类
医药卫生引用本文复制引用
崔京福,徐耀增,朱世军,朱锋,付稳,邵洪国,耿德春..淫羊藿苷抑制钛颗粒诱导的炎症反应[J].中国组织工程研究,2014,(16):2563-2569,7.基金项目
江苏省临床医学科技专项资助项目(BL2012004)；国家自然科学基金资助项目(81101399，81272018，81372018)；江苏省自然科学基金资助项目(BK2011303)；江苏省研究生培养创新工程资助项目(CXZZ13_0835) （BL2012004）
