中国组织工程研究Issue(18)：2795-2800,6.DOI:10.3969/j.issn.2095-4344.2014.18.002
大鼠胫骨标准实验性骨不连模型的制作
Establishment of an experimental tibial nonunion model in rats
房国军 1曲志国 1崔正宏 1刘英2
作者信息
- 1. 吉林省四平市中心医院骨科，吉林省四平市 136000
- 2. 吉林省四平市中心医院干细胞实验中心，吉林省四平市 136000
- 折叠
摘要
Abstract
BACKGROUND:Establishment of an objective and standard experimental animal model of bone non-union is necessary for experimental study and related treatment of non-union.
<br> OBJECTIVE:To establish an objective experimental animal model for the experimental study of non-union.
<br> METHODS:Sprague-Dawley rats were included in this study, the middle tibia was cut off, and fracture fragments (including periosteum) were seared with dental cement plugger (3# or 4#). Subsequently, bone marrow cavity was dril ed using a 0.5 mm Kirschner wire, and the fractures were fixed. 2 months later, the specimens were detected by gross observation, histopathological detection and radiological examination to determine the nonunion formation.
<br> RESULTS AND CONCLUSION:The results of gross observation, histopathological detection and radiological examination showed that, no bony union was found at bone defect area, which was fil ed by fiber scar tissue, and bone end sclerosis was apparent. The animal models established in this study have pathological changes of bone non-union, which is in line with the requirements of bone non-union, so the model can be considered a reliable and practical animal model of bone non-union.关键词
实验动物/组织构建/胫骨/骨不连/模型/放射学/病理学Key words
tibia/fractures/bone/models/animal分类
医药卫生引用本文复制引用
房国军,曲志国,崔正宏,刘英..大鼠胫骨标准实验性骨不连模型的制作[J].中国组织工程研究,2014,(18):2795-2800,6.
