中国组织工程研究Issue(18)：2801-2805,5.DOI:10.3969/j.issn.2095-4344.2014.18.003
转导骨桥蛋白短发夹状RNA预防动脉粥样硬化模型兔血管成形后的再狭窄
Transduction of osteopontin short hairpin RNA in prevention of restenosis after angioplasty in a rabbit model of atherosclerosis
摘要
Abstract
BACKGROUND:Restenosis after angioplasty severely limited the application and long-period therapeutic effects of percutaneous coronary intervention. Changes in smooth muscle cel phenotype and their proliferation are important mechanisms of restenosis after angioplasty. <br> OBJECTIVE:To use bal oon in vivo transduction of osteopontin short hairpin RNA (OPN-shRNA), to inhibit osteopontin expression at the injured blood vessels of a rabbit model of experimental atherosclerosis, and to prevent restenosis after angioplasty. <br> METHODS:A total of 20 rabbit models of atherosclerosis were established and randomly equal y assigned to empty plasmid group and OPN-shRNA plasmid group. The plasmid recombinant OPN-shRNA and empty plasmid were transferred to the ventral aorta by bal oon. <br> RESULTS AND CONCLUSION:After bal oon dilatation, specific green fluorescence was detected in the layer of vascular smooth muscle in the two groups. Moreover, with prolonged time of transfection, fluorescence intensity gradual y decreased. Compared with the empty plasmid group, the expanded artery lumen area obviously increased in the OPN-shRNA plasmid group, and plaque burden evidently reduced. Results indicated that bal oon catheter used in regional blood vessels in rabbit models of atherosclerosis could successful y transduce OPN-shRNA plasmid. The restenosis of the expanded blood vessels lessened, and thrombus burden relieved. It is of great importance to prevent the occurrence of restenosis after angioplasty in rabbit models.关键词
动物模型/组织工程/RNA干扰/短发卡样RNA/骨桥蛋白/再狭窄/基因治疗/动脉粥样硬化/血管成形/平滑肌细胞Key words
atherosclerosis/coronary artery disease/RNA interference/osteopontin/myocytes/smooth muscle分类
医药卫生引用本文复制引用
孙玉梅,张金盈,闫继锋,袁斌,杨鹏伟,李文,于运福..转导骨桥蛋白短发夹状RNA预防动脉粥样硬化模型兔血管成形后的再狭窄[J].中国组织工程研究,2014,(18):2801-2805,5.基金项目
湖北省科技攻关课题(2006AA301C18)Funding:the Hubei Provincial Science and Technology Project, No.2006AA301C18 （2006AA301C18）
