中国组织工程研究Issue(18)：2886-2891,6.DOI:10.3969/j.issn.2095-4344.2014.18.017
制备心肌梗死大鼠模型方法的改进
Modified method of establishing a rat model of myocardial infarction
赵传艳 1王昕2
作者信息
- 1. 潍坊市益都中心医院心内二科，山东省潍坊市 262500
- 2. 青州市人民医院，山东省青州市 262500
- 折叠
摘要
Abstract
BACKGROUND:An animal model of myocardial infarction is of great significance for the study of the pathogenesis, pathophysiology and evaluation of treatment methods of human coronary artery disease. Many researchers aim to improve survival rate and the success rate of model induction. <br> OBJECTIVE:To improve the method of making a rat model of myocardial infarction. <br> METHODS:One hundred Wistar rats were divided into model group (n=80) and sham-operated group (n=20). The rats were intraperitoneal y anesthetized with 3%sodium pentobarbital. Tracheostomy was operated. An automatic breathing apparatus was utilized for assisted breathing. A left anterior thoracotomy was performed at the fourth intercostal space. The left anterior descending coronary artery was ligated. Sham-operated rats underwent similar surgery but without coronary artery ligation. The thoracic cavity was closed layer by layer. The breathing apparatus was removed and tracheal intubation was also removed. To prevent suffocation, the trachea and the neck incision were not closed. <br> RESULTS AND CONCLUSION:Heart morphology and hematoxylin-eosin staining demonstrated that the success rate of establishing myocardial infarction model was 98.6%. The survival rate of model group at 3 weeks after surgery was 88.75%. Total y nine rats died, including seven dead rats during operation and within 24 hours after operation, and two dead rats in later stage. The survival rate of the sham-operated group was 100%. We modified the method of establishing a rat model of myocardial infarction and improved the operation success rate and survival rate of rats, such as anesthesia method, longitudinal incision during tracheotomy, without closing the trachea after operation, and the method of opening and closing the chest.关键词
实验动物/组织构建/心肌梗死/大鼠/存活率/成功率/改进Key words
myocardial infarction/models, animal/rats/Wistar/survival rate分类
医药卫生引用本文复制引用
赵传艳,王昕..制备心肌梗死大鼠模型方法的改进[J].中国组织工程研究,2014,(18):2886-2891,6.
