中国组织工程研究Issue(19)：2961-2967,7.DOI:10.3969/j.issn.2095-4344
miR-378对缺血缺氧条件下骨髓间充质干细胞增殖及凋亡的影响
miR-378 effects on the proliferation and apoptosis of bone marrow mesenchymal stem cells under hypoxic-ischemic condition
摘要
Abstract
BACKGROUND:To investigate the influence of miR-378 on bone marrow mesenchymal stem cels proliferation and apoptosis under hypoxic-ischemic condition wil provide a new method for further improving the survival of bone marrow mesenchymal stem cels in the infarcted myocardium. <br> OBJECTIVE:To observe the viability of bone marrow mesenchymal stem cels and their ability of angiogenesis under hypoxic-ischemic condition after miR-378 transfection. <br> METHODS:The bone marrow mesenchymal stem cels of Sprague-Dawley rat were culturedin vitro and divided into the non-transfection group and the transfection group. Mesenchymal stem cels were transfected with miR-378 mimic in the transfection group while the non-transfection group was taken as the control group. After transfection, the two groups were incubated under hypoxic-ischemic condition (serum-free medium,1% O2, 5% CO2, 94% N2) at 37℃ for 24 hours. Viability and proliferation of bone marrow mesenchymal stem cels were evaluated by the trypan-blue exclusion assay and MTS assay respectively. The cellapoptosis was detected by TUNEL assay. The culture supernatant of the two groups was colected separately and used as the conditioned medium after their exposure to hypoxic-ischemic environment. Endothelial cels were then co-cultured with the conditioned medium for the vasculature formation assay. <br> RESULTS AND CONCLUSION: After the experience of hypoxia-ischemia for 24 hours and 72 hours, there was a larger number of the living cels in the transfection group in contrast to the non-transfection group (bothP < 0.01). Compared with the non-transfection group, miR-378-MSCs group presented a stronger ability of proliferation, and their proliferation rates were obviously higher at 24 and 48 hours (P < 0.01 andP < 0.05, respectively). The percentage of apoptotic cels in the transfection group was significantly declined under the hypoxic-ischemic condition (P < 0.05). The vasculature formation assay indicated that the lumen-like structures were found in both of the two groups, however, the number of lumen-like structures was remarkably increased in the transfection group (P < 0.01). miR-378 could promote the proliferation of bone marrow mesenchymal stem cels and suppress their apoptosis under hypoxic-ischemic condition. It could also enhance their ability of vasculogenesis.关键词
干细胞/骨髓干细胞/骨髓间充质干细胞/miR-378/缺血缺氧/增殖/凋亡/血管形成能力/国家自然科学基金Key words
bone marrow/mesenchymal stem cels/microRNAs/ischemia/anoxia/cellproliferation/apoptosis分类
医药卫生引用本文复制引用
郭天柱,邢越,侯婧瑛,周长青,钟婷婷,郑韶欣,王彤..miR-378对缺血缺氧条件下骨髓间充质干细胞增殖及凋亡的影响[J].中国组织工程研究,2014,(19):2961-2967,7.基金项目
国家自然科学基金(81070125)“抗凋亡与促血管生成miRNA-378干预MSCs治疗心梗后心衰的机制研究”；国家自然科学基金(81270213)“ANGⅡ/AT1/SMAD/CX43通路在心肌干细胞提高心梗大鼠心电生理学稳定性和室颤阈值的作用机制研究”；广东省科技计划项目(2010B031600032)“抗凋亡与促血管生成miRNA-378干预MSCs治疗心梗后心衰的机制研究”；高校基本科研业务费中山大学青年教师重点培育项目(13ykzd16)“PPAR-γ/TGF-β1/Smad/CX43通路在PPAR-γ干预MSCs治疗心梗后心衰的疗效及机制研究” （81070125）
