中国组织工程研究Issue(20)：3236-3241,6.DOI:10.3969/j.issn.2095-4344.2014.20.022
骨形态发生蛋白2诱导C2C12和MC3T3-E1的成骨分化与自噬
Bone morphogenetic protein 2-induced C2C12 and MC3T3-E1 osteoblast differentiation and autophagy
摘要
Abstract
BACKGROUND:A series of studies indicate that autophagy is closely linked with differentiation. Bone morphogenetic protein 2 (BMP-2) is the classical pathway for C2C12 and MC3T3-E1 osteoblast differentiation, and the ideal model to study osteogenic differentiation process. <br> OBJECTIVE:To observe the relationship between autophagy and BMP-2-induced celllines C2C12, MC3T3-E1 osteoblast differentiation. <br> METHODS:Real-Time PCR was applied to detect osteogenic differentiation and autophagy related index after C2C12 and MC3T3-E1 were induced with BMP-2 (100μg/L) for 72 hours. The osteogenic index alkaline phosphatase in BMP-2-induced MC3T3-E1 and C2C12 cultured with different concentrations of 3-methyladenine (0, 1, 5, 10 mmol/L) was determined with alkaline phosphatase staining. Western blot analysis was applied to detect LC3-I/II expression levels in C2C12 and MC3T3-E1 induced with BMP-2 for different time points (0, 12, 24, 48, 72, 96 hours). <br> RESULTS AND CONCLUSION:The autophagy-related mRNA and protein expression showed an increasing tendency and autophagy-related protein LC3 levels was increased, which was associated with the time, during the BMP-2-induced celllines C2C12, MC3T3-E1 osteoblast differentiation. Meanwhile, alkaline phosphatase expression levels were inhibited by autophagy in the process of osteogenic differentiation. Therefore, there is a close relationship between autophagy and the BMP-2-induced celllines C2C12, MC3T3-E1 osteoblast differentiation.关键词
组织构建/骨组织工程/自噬/骨形态发生蛋白2/C2C12/MC3T3-E1/成骨分化/国家自然科学基金Key words
autophagy/bone morphogenetic proteins/cellline/myoblasts/osteoblasts分类
医药卫生引用本文复制引用
高艳,程晨,李静,张越,肖伟凡,潘秋辉..骨形态发生蛋白2诱导C2C12和MC3T3-E1的成骨分化与自噬[J].中国组织工程研究,2014,(20):3236-3241,6.基金项目
国家自然科学基金项目(81171178) （81171178）
