中国组织工程研究Issue(20)：3242-3245,4.DOI:10.3969/j.issn.2095-4344.2014.20.023
周期性张应力对C2C12成肌细胞增殖的影响
Effect of cyclic stretch on the proliferation of C2C12 myoblasts
摘要
Abstract
BACKGROUND:Mechanical stimulation triggers a muscle tissue remodeling, during which, myoblast process is a multi-stage development process. Many signals in extracellular matrix are involved in myoblast process, and mechanical signals are considered as important external factors for muscle formation and regeneration. <br> Numerous studies concerning cyclic tensile stress effects on myoblast proliferation and apoptosis have been reported, but the specific mechanism underlying the mechanical stimulation in muscle formation is stil unclear. OBJECTIVE:To study the effects of cyclic stretch on proliferation of C2C12 myoblasts and its mechanism. <br> METHODS:C2C12 myoblasts were cultured in vitro under 10%cyclic stretch for 6, 12 and 24 hours. <br> RESULTS AND CONCLUSION:The results of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that with the time of cyclic stretch, the proliferation of C2C12 myoblasts and the rate of S-phase fraction were gradual y increased and peaked at 12 hours (P<0.05), and then decreased. Western blot assay showed that the expression of nuclear transcription factorκB protein was decreased with time, which was inversely correlated to the proliferation of C2C12 myoblasts, but increased at 24 hours under the cyclic stretch. These findings indicate that the cyclic stretch can induce C2C12 myoblasts proliferation and alter the cellcycle. Nuclear transcription factorκB may be involved in this process.关键词
组织工程/骨组织工程/组织构建基础实验/张应力/周期性/C2C12成肌细胞/增殖/核转录因子κB/国家自然科学基金Key words
myoblasts/periodicity/elasticity/cellcount/cells分类
医药卫生引用本文复制引用
王洪玲,刘东旭,袁晓..周期性张应力对C2C12成肌细胞增殖的影响[J].中国组织工程研究,2014,(20):3242-3245,4.基金项目
国家自然科学基金(30871426) （30871426）
