中国组织工程研究Issue(21)：3299-3303,5.DOI:10.3969/j.issn.2095-4344.2014.21.004
不同接种方式对组织工程牙龈种子细胞黏附效果的影响
Effects of different implant methods on in vitro adhesion of tissue-engineered gingival cells
摘要
Abstract
BACKGROUND:How to construct tissue-engineered periodontal tissue through efficient combination of scaffold materials and seed cells in vitro is an important direction of current research. <br> OBJECTIVE:To compare the cellseeding efficiency of traditional static seeding method and col agen enclose seeding method to select a more appropriate seeding method for cellattach. <br> METHODS:Gingival fibroblasts from dogs at certain concentrations were seeded onto polylactic acid-chitosan-gelatin scaffolds with gradient and uniform pore sizes using the traditional static seeding method and col agen enclose seeding method, respectively. The number of adherent cells was counted to calculate the seeding efficiency for data comparison. RESULTS AND CONCLUSION:The cellseeding efficiency of the col agen enclose seeding method was higher than that of the traditional static seeding method (P<0.01). The col agen enclose seeding method can significantly improve the seeding efficiency and increase cellinitial concentration on the scaffold, and this method can be used for gingival tissue engineering.关键词
生物材料/口腔生物材料/口腔组织工程/种子细胞/沉淀接种法/胶原包裹接种法/聚乳酸-壳聚糖-明胶/细胞接种率/牙龈成纤维细胞Key words
vaccination/gingival/cells/celladhesion/stents/fibroblasts分类
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钟代琴,周勤虎,靳趁心,梁妍..不同接种方式对组织工程牙龈种子细胞黏附效果的影响[J].中国组织工程研究,2014,(21):3299-3303,5.基金项目
天津市高等学校科技发展基金项目(20110140) （20110140）
