中国组织工程研究Issue(23)：3653-3659,7.DOI:10.3969/j.issn.2095-4344.2014.23.008
骨髓间充质干细胞旁分泌对急性心肌梗死心肌的保护作用
Bone marrow mesenchymal stem cells protect myocardial function in acute myocardial infarction through a paracrine mechanism
邹松平 1王宇 1李春雨 1付雪丹 1刘彦丽1
作者信息
- 1. 大庆龙南医院物理诊断科，黑龙江省大庆市 163453
- 折叠
摘要
Abstract
BACKGROUND:Adult cardiomyocytes show no regenerative ability, and celltherapy for myocardial regeneration and repair may improve myocardial ischemic injury function. OBJECTIVE:To confirm the effect and reveal the mechanism of bone marrow mesenchymal stem cells (BMSCs) on acute myocardial infarction (AMI). METHODS:BMSCs were isolated, cultured from bone marrow of Sprague-Dawley rats using density gradient centrifugation. AMI models were produced in 20 rats by ligating the left anterior descending (LAD) coronary artery, and randomly divided into model group and BMSCs group. In the BMSCs group, cells were subsequently injected with a sterile microinjection via the tail vein. RESULTS AND CONCLUSION:Six months postoperatively, the cardiac function was improved, the vessel density was increased, the percentage of apoptotic cells was decreased in the BMSCs group than that in the model group;the expression levels of inflammatory factors, including vascular endothelial growth factor, von Wil ebrand factor, transforming growth factor 3β, and interleukin-1βmRNA were significantly improved in the BMSCs group than that in the model group. These results showed that BMSCs can protect the myocardium from AMI by regulating the secretion of inflammatory cytokines and angiogenic factors.关键词
干细胞/移植/急性心肌梗死/骨髓间充质干细胞/干细胞移植/旁分泌/心脏功能/血管密度/细胞凋亡/炎症因子Key words
myocardium/myocardial infarction/mesenchymal stem cells/mesenchymal stem celltransplantation/intercellular signaling peptides and proteins分类
医药卫生引用本文复制引用
邹松平,王宇,李春雨,付雪丹,刘彦丽..骨髓间充质干细胞旁分泌对急性心肌梗死心肌的保护作用[J].中国组织工程研究,2014,(23):3653-3659,7.
