中国组织工程研究Issue(23)：3715-3722,8.DOI:10.3969/j.issn.2095-4344.2014.23.018
小鼠心肌祖细胞的永生化及筛选
Immortalization and screening of cardiac progenitor cells in mice
摘要
Abstract
BACKGROUND:Transplantation of exogenous stem cells for functional cardiac celldeath or apoptosis easily induced complications after transplantation. Therefore, cardiac progenitor cells of heart itself have been ideal seed cells. OBJECTIVE:To establish stable celllines of cardiac progenitor cells from mouse heart, and to provide ideal cellmodels for studying proliferation and differentiation factors affecting cardiac progenitor cells during adult myocardial cells were damaged. METHODS:(1) Myocardiocytes were isolated from embryonic 15.5 days mice. (2) The cultured myocardiocytes were immortalized using retrovirus SSR69. Immortalized monoclonal myocardial cells were obtained using antibiotic selection and infinite dilution. (3) Monoclonal cellline with the property of cardiac progenitor cells was screened out. RESULTS AND CONCLUSION:(1) Myocardiocytes were successful y isolated and cultured. Partial cells showed obvious beating. (2) Myocardiocytes infected with retrovirus SSR69 were cloned and got 76 clones, then were named as CP15-#, (3) Screening the first 20 clones, the reasonable clones with the characteristics of cardiac progenitor cells were obtained according to myocardial cellmarker genes. The results suggested that immortalized cardiac progenitor cells were established mediated by reversible SV40 T antigen.关键词
干细胞/培养/组织构建/心肌祖细胞/心肌细胞/永生化/SSR69/国家自然科学基金Key words
myocytes, cardiac/clone cells/genes分类
医药卫生引用本文复制引用
朱高慧,何通川,熊丰,朱岷,陈沅..小鼠心肌祖细胞的永生化及筛选[J].中国组织工程研究,2014,(23):3715-3722,8.基金项目
国家自然科学基金面上项目(30772311)；重庆市科委自然科学基金(CSTC,2010BB5107)Funding:the National Natural Science Foundation of China, No.30772311 （30772311）
the Natural Science Foundation of Science Committee of Chongqing City, No. CSTC 2010BB5107 （）
