中国组织工程研究Issue(23)：3723-3726,4.DOI:10.3969/j.issn.2095-4344.2014.23.019
人牙髓干细胞的体外培养及神经样诱导分化
In vitro culture and neuron-like differentiation of human dental pulp stem cells
方成志 1杨于嘉 2姚跃 1何学华1
作者信息
- 1. 中南大学附属湘雅医院儿科，湖南省长沙市 410013
- 2. 武汉市儿童医院新生儿科，湖北省武汉市 430016
- 折叠
摘要
Abstract
BACKGROUND:The discovery and concept of pulp tissue-derived stem cells is beneficial to the understanding of tooth development and regeneration and repair mechanisms from the cellular level. OBJECTIVE:To understand the induced differentiation capacity and induced conditions in vitro of human dental pulp stem cells into neuron-like cells. METHODS:Pulp tissue was separated from human healthy third molars. Single cellsuspensions were prepared and seeded into 6-wel plates containing alpha-modified minimum essential medium supplemented with 15%fetal bovine serum. Subconfluent cultures (first passage) of colony forming cells were induced with butylhydroxy anisole, forskolin,β-mercaptoethanol, basic fibroblast growth factor. RESULTS AND CONCLUSION:Immunofluorescence and reverse transcription-PCR assay showed that human dental pulp stem cells positively expressed stro-1, Col-I, dentin sialoprotein after 2 weeks of induction. Nestin and neuron-specific enolase were strongly expressed, but the gingival fibroblasts were negatively expressed. It indicates that adult stem cells in human dental pulp have a high neuron-like celldifferentiation potential under a certain inductive condition.关键词
干细胞/培养/干细胞培养与分化/人牙髓细胞/免疫表型/多向分化Key words
stem cells/dental pulp/celldifferentiation/immunophenotyping分类
医药卫生引用本文复制引用
方成志,杨于嘉,姚跃,何学华..人牙髓干细胞的体外培养及神经样诱导分化[J].中国组织工程研究,2014,(23):3723-3726,4.
