中国组织工程研究Issue(23)：3732-3738,7.DOI:10.3969/j.issn.2095-4344.2014.23.021
长链非编码RNA AK089560在间质干细胞成骨与成脂分化中的表达
The expression of long non-coding RNA AK089560 in mesenchymal stem cells undergoing osteogenic and adipogenic differentiation
摘要
Abstract
BACKGROUND:Recent studies have found that stem cellpluripotency and differentiation is regulated by many long non-coding RNAs (LncRNAs). The expression and effect of LncRNA AK089560 during differentiation of stem cells is unclear. OBJECTIVE:To investigate the expression of LncRNA AK089560 in mesenchymal stem cells C3H10T1/2 undergoing osteogenic and adipogenic differentiation. METHODS:Osteogenic differentiation of mesenchymal stem cells C3H10T1/2 was induced by recombinant human bone morphogenetic protein-2 and evaluated using alkaline phosphatase staining. The adipogenic differentiation of mesenchymal stem cells C3H10T1/2 was induced by three factors (dexamethasone, indomethacin and insulin) and evaluated by oil red O staining. The dynamical expression of LncRNA AK089560 was detected by qRT-PCR assay. The AK089560 secondary structure was predicted using RNAfold software. The relationship between AK089560 and neighboring protein-coding genes was analyzed using UCSC genome browser and visualized by fancyGENE online software. RESULTS AND CONCLUSION:Over 70%of C3H10T1/2 cells were positive for alkaline phosphatase after osteogenic induction and more than 80%of the cells positive for oil red O staining after adipogenic induction. qRT-PCR results showed that the expression of LncRNA AK089560 at days 2, 4, 6 of both osteogenic and adipogenic differentiation was significantly decreased compared with the control group (P<0.05). Bioinformatics analysis showed that there was a stem-loop structure for AK089560 and sense overlap relationship between AK089560 and protein-encoding gene Sema3a. These findings indicate that LncRNA AK089560 expression is reduced during osteogenic differentiation and adipogenic differentiation, showing that AK089560 may be involved in regulating the multi-directional differentiation of stem cells.关键词
干细胞/分化/间质干细胞/成骨分化/成脂分化/长链非编码RNA/生物信息学/重组人骨形态发生蛋白2/国家自然科学基金Key words
celldifferentiation/mesenchymal stem cells/bone morphogenetic proteins分类
医药卫生引用本文复制引用
左长清,卢旱云,钟月春,汪宗桂,戴忠,刘钰瑜,吴铁..长链非编码RNA AK089560在间质干细胞成骨与成脂分化中的表达[J].中国组织工程研究,2014,(23):3732-3738,7.基金项目
国家自然科学基金项目(81101357，81102450)；东莞市高等院校科研机构科技项目(2011108102029)Funding:the National Natural Science Foundation of China, No.81101357,81102450 （81101357，81102450）
Science and Technological Program for Dongguan’s Higher Education, Science and Research, and Health Care Institutions，No.2011108102029 （）
