中国组织工程研究Issue(27)：4362-4367,6.DOI:10.3969/j.issn.2095-4344.2014.27.016
面神经损伤模型中的半胱氨酸天冬氨酸蛋白酶相关蛋白表达与损伤相关性
Correlation between caspase regulatory gene expression and facial nerve injury in a facial nerve injury model
摘要
Abstract
BACKGROUND:Caspase plays a crucial role in the cellapoptosis, but the influence of different facial nerve injury on caspase 1, caspase 8, cyto-c protein expression and their correlation stil remain unclear. <br> OBJECTIVE:To construct facial nerve crush or distal transection injury models, observe the morphological changes of facial motoneurons, investigate death gene caspase 3, caspase 8, cyto-c expression, and analyze their correlation. <br> METHODS:Facial nerve crush or distal transection injury model was established in the right facial nerve of rats, while the left facial nerve served as normal controls. We observed the morphology and the death of facial motoneurons with toluidine blue staining and transmission electron microscope. Expressions of caspase 3, caspase 8 and cyto-c proteins were studied by immunohistochemistry analysis fol owing facial nerve injury. <br> RESULTS AND CONCLUSION:Both facial nerve distal transection and crush injury resulted in the death of facial motoneurons, and the death pattern was mainly apoptosis. Caspase 3, caspase 8 and cyto-c protein expressions were observed in the subnucleus of normal rat facial nucleus. cells of the distal transection group were stained more intensely than that of crush group. Expressions of these proteins began to increase at 3 days after the injuries. Caspase 3 and caspase 8 protein expression peaked at 14 days, whereas cyto-c protein expression peaked at 7 days after the injuries. Expressions of caspase 3, caspase 8 and cyto-c proteins were correlated with facial nerve injury type and injury time. Expressions of caspase 8 and cyto-c protein were correlated with expression of caspase 3 protein. The findings indicate that, caspase 8 and cyto-c contribute to activate caspase 3, and caspase cascade reaction plays an important role in the apoptosis of facial motoneurons.关键词
实验动物/组织构建/面神经/损伤/细胞死亡/调控基因/半胱氨酸天冬氨酸蛋白酶Key words
facial nerve/cell death/caspases/gene分类
医药卫生引用本文复制引用
魏海刚,李蜀光,陈玉婷,蔡超雄,许彪..面神经损伤模型中的半胱氨酸天冬氨酸蛋白酶相关蛋白表达与损伤相关性[J].中国组织工程研究,2014,(27):4362-4367,6.基金项目
广东省医学科学技术研究基金资助(B2003144)；佛山市科技局医学专项基金立项(200508062)Funding:Medical Science and Technology Research Fund of Guangdong Province, No. B2003144 （B2003144）
Medical Foundation by Foshan Municipal Science and Technology Bureau, No.200508062 （）
