中国组织工程研究Issue(27)：4375-4382,8.DOI:10.3969/j.issn.2095-4344.2014.27.018
动脉粥样硬化并肢体缺血模型兔接受低频电磁场刺激促进新生血管形成
Low-frequency magnetic fields promote neovascularization in a rabbit model of atherosclerosis combined with limb ischemia
摘要
Abstract
BACKGROUND:Studies on basic research of magnetic treatment of limb ischemic disease are not much, because poor compliance of animals and the stability of the magnetic field strength are difficult to control, resulting in big experimental error and decreased credibility of the results. For this kind of problem, experimental study on low-frequency electromagnetic magnetic cages for treatment of ischemic limbs was conducted, thus overcoming the two major issues of poor compliance of animals and difficult control of the stability of magnetic field strength. <br> OBJECTIVE:To investigate the effects of self-made low-frequency magnetic fields of rabbit cages on neovascular growth-promoting factor of rabbits with limb ischemia. <br> METHODS:A total of 96 rabbit models of atherosclerosis were constructed, numbered and randomly divided into ischemia group and non-ischemia group (12 treatment combination in each group). Experiments in each group were performed four times according to the requirement of factorial design. Electromagnetic field intensity factor A (0, 3, 6, 12 mT) and the time factor B (3, 5, 7 days) were set. <br> RESULTS AND CONCLUSION:Low-frequency magnetic field could apparently promote hypoxia inducible factor-1α, vascular endothelial growth factor and CD34 expression in ischemic limb of rabbits. Electromagnetic field intensity factor A was a key factor for contributing to the expression of hypoxia inducible factor-1α, vascular endothelial growth factor and CD34, and the time factor B was secondary factor. Low-frequency magnetic field also promoted hypoxia inducible factor-1αexpression in non-ischemia limb, but did not promote vascular endothelial growth factor and CD34 expression. Thus, the expression of vascular endothelial growth factor and CD34 was regulated by hypoxia inducible factor-1α, as wel as other factors, in the ischemic state.关键词
实验动物/组织构建/磁性饲养笼/低频电磁场/家兔/肢体缺血/缺氧诱导因子1α/血管内皮生长因子/CD34/新疆维吾尔自治区自然科学基金Key words
electromagnetic fields/atherosclerosis/ischemia/muscle, striated/hypoxia-inducible factor 1, alphasubunit/vascular endothelial growth factors分类
医药卫生引用本文复制引用
张建勇,彭辉,孟德凯,陈辉,张运强,余化龙..动脉粥样硬化并肢体缺血模型兔接受低频电磁场刺激促进新生血管形成[J].中国组织工程研究,2014,(27):4375-4382,8.基金项目
新疆维吾尔自治区自然科学基金资助(2011211A093)Funding:the Natural Science Foundation of Xinjiang Uygur Autonomous Region, No.2011211A093 （2011211A093）
