中国组织工程研究Issue(27)：4406-4411,6.DOI:10.3969/j.issn.2095-4344.2014.27.023
比较2种常用授精法在体外授精-胚胎移植中的差异
Comparison of two common insemination methods on in-vitro fertilization and embryo transfer
摘要
Abstract
The in-vitro fertilization and embryo transfer technique has been widely applied in human insemination. The rate of successful insemination is gradual y rising, and the in-vitro fertilization directly determine the insemination outcome. <br> OBJECTIVE:To evaluate the difference between the two common using insemination methods, microdrop and open, in in-vitro fertilization and embryo development. <br> METHODS:A randomized study was conducted to compare microdrop and open insemination methods among non-male factor patients undergoing in-vitro fertilization and embryo transfer. A total of 1 175 cases were enrol ed in the research. There were 573 cases in the microdrop group, and 602 cases in open insemination group. The fertilization rate and embryo development in the two groups were compared. <br> RESULTS AND CONCLUSION:The fertilization failure rate [total fertilization failure rate+low fertilization rate (<25%oocytes fertilized)] in the microdrop insemination group was higher than in the open insemination group (11.9%, 3.3%, P<0.001), while the good quality embryo rate and pregnancy rate did not differ significantly between the two groups (al P>0.05). The open insemination method is a simple insemination method with a lower fertilization failure rate. As the fertilization is a highly complicated process involving many extrinsic and intrinsic factors, further study is needed to confirm the effects of the two insemination methods on in-vitro fertilization outcome.关键词
组织构建/精子/授精/卵子/体外授精/受精失败/妊娠率/国家自然科学基金Key words
sperm/fertilization, in vitro/pregnancy rate分类
医药卫生引用本文复制引用
罗灿峤,李宇彬,李涛,肖珊,龙玲莉,谢文林,聂钊铭..比较2种常用授精法在体外授精-胚胎移植中的差异[J].中国组织工程研究,2014,(27):4406-4411,6.基金项目
国家自然科学青年基金(81100470，81270750)；中山大学青年教师培育项目(12ykpy24)；广东省自然科学基金资助项目(S2012010009390)Funding:the National Natural Science Foundation of China, No.81100470,81270750 （81100470，81270750）
Young Teachers Cultivation Project of Sun Yat-sen University, No.12ykpy24 （）
the Natural Science Foundation of Guangdong （）
