中国组织工程研究Issue(30)：4771-4776,6.DOI:10.3969/j.issn.2095-4344.2014.30.003
电纺丝技术制备组织工程食管仿生支架
Preparation of tissue-engineered esophageal scaffolds using electrospinning technology
摘要
Abstract
BACKGROUND:We have found that oriented fibers can guide the alignment of smooth muscle cells in our previous experiments. Thus, we designed the experiment to prepare wel aligned polymeric fibers using electrospinning technology, aiming at guiding the growth of esophageal smooth muscle cells to maintain cellmorphology and biological function. OBJECTIVE:Using electrospinning technology, to fabricate isotropic and directed nano-fibrous scaffolds made of polycaprolacton, gelatin and silk fibroin. METHODS:Polycaprolacton/silk fibroin fibers at a ratio of 4:1 were prepared with proper parameters, including solution concentration, voltage and injection speed, under the self-made spinning system. The polycaprolacton/gelatin sheets with mass ratio of 2:1, 1:1 and 1:2, respectively, were also fabricated under suitable process parameters. Using the rol er col ector instead of the metal plate, polycaprolacton/gelatin nano-fibrous scaffold with good alignment of fibers was manufactured. RESULTS AND CONCLUSION:The isotropic polycaprolacton/silk fibroin scaffold with fiber diameter of (535.9±126.7) nm was prepared under conditions of solution concentration (0.08 g/mL), injection speed (1.6 mL/h) and voltage (22.5 kV), and these fibers were uniform with no beads. The isotropic polycaprolacton/gelatin scaffold with fiber diameter of (257.9±117.8) nm was prepared under conditions of solution concentration (0.10 g/mL), injection speed (0.8 mL/h) and voltage (22.5 kV). Using the rol er col ector instead of the previous metal plate, polycaprolacton/gelatin (w:w, 1:2) nano-fibrous scaffold with good alignment of fibers was manufactured. The process parameters were 3 000 r/min of rol ing speed, 0.8 mL/h of injection speed and 15 kV of voltage.关键词
生物材料/纳米材料/电纺丝/聚己内酯/纳米纤维/取向/食管组织工程/仿生支架/国家自然科学基金Key words
nanofibers/gelatin/silk分类
医药卫生引用本文复制引用
於学婵,沈秋霞,卢珍珍,张陈,邓玲,胡品,竺亚斌..电纺丝技术制备组织工程食管仿生支架[J].中国组织工程研究,2014,(30):4771-4776,6.基金项目
国家自然科学基金(81171476)；宁波市创新团队项目(2011B82014)；宁波市社发项目(2013C50031)@@@@the National Natural Science Foundation of China,81171476 （81171476）
the Innovative Team Program of Ningbo City, No.2011B82014 （）
the Social Development Project of Ningbo City, No.2013C50031 （）
