中国组织工程研究Issue(30)：4816-4822,7.DOI:10.3969/j.issn.2095-4344.2014.30.010
铍对牙龈卟啉单胞菌菌毛基因型的影响
Influence of beryllium ions on genotype of Porphyromonas gingivalis fimbriae gene cluster
摘要
Abstract
BACKGROUND:In oral warm and moisture circumstance, al oy which contains Be is easily to be eroded to release Be2+. But there is stil no research focusing on beryl ium influence on genotype of Porphyromonas gingivalis fimbriae gene cluster. OBJECTIVE:To investigate Be 2+effect on genotype of Porphyromonas gingivalis fimbriae gene cluster. METHODS:The revived Porphyromonas gingivalis was resuscitated for 48 hours in the anaerobic culture medium with different concentration of Be 2+(10×10-6, 5×10-6, 1.25×10-6). Through PCR amplification and sequencing, we investigated the effects of Be2+RESULTS AND CONCLUSION:(1) When Be on genotypes of Porphyromonas gingivalis fimbriae gene cluster. 2+concentration was 5×10-6, we found the peak of 217 and 257 sites on DNA sequence expressing G/A overlap peak, different from G single peak of the other groups, suggesting the suspicious bases changes, part of the single base G mutated into A. (2) On al concentrations, we found a base group composed of seven A bases was inserted into the 101 site of DNA sequence. Up to now, there is no direct contacts of the mutations occurring to Be2+concentration. Changes of gene may lead to the shifting of the reading frame, the abnormal synthesis of proteins, the change of Porphyromonas gingivalis fimA gene toxicity, and lastly the unbalance of the micro-ecological environment.关键词
生物材料/口腔生物材料/牙周损伤/铍离子/牙龈卟啉单胞菌/菌毛/黏附能力/致病力/fimA分型Key words
beryl ium/Porphyromonas gingivalis/fimbriae, bacterial/pathogenicity分类
医药卫生引用本文复制引用
黄睿,李庆艳,林华,郭巍,高宁..铍对牙龈卟啉单胞菌菌毛基因型的影响[J].中国组织工程研究,2014,(30):4816-4822,7.基金项目
四川省科技厅支持项目(2008SG0023)@@@@the Grant from Sichuan Science and Technology Bureau, No.2008SG0023 （2008SG0023）
