中国组织工程研究Issue(30)：4863-4867,5.DOI:10.3969/j.issn.2095-4344.2014.30.017
不同脱细胞方式下所得胞外基质存在的差异
Effect of different decellularizing methods on cell-secreted extracellular matrix in vitro
摘要
Abstract
BACKGROUND:Compared with single or composite biomolecular materials, decellulazied matrices are more biomimetic to natural ectocytic surroundings. So cel-secreted extracellular matrix is paid more and more attention in the field of tissue engineering, and the composition of these matrices are influenced by decellulezired preparation methods more or less. But there are few studies about the biological effect of different decellulazried methods on the extracellular matrix. OBJECTIVE:To investigate the composition of cel-secreted extracellular matrices in vitro by different decellularizing methods and their effects as surface modification on cytobiological reaction. METHODS:After the treatment of different decellularizing methods (freeze/thaw cycles, trypsin, weak alkal, detergents), the extracellular matrix was obtained and grouped into four kinds. The biological composition of the extracellular matrix was determined by ELISA assay. Then osteoblasts were seeded onto the four kinds of extracellular matrix surfaces. cells cultured normal y served as controls. The effect of extracellular matrix coatings on cellgrowth and differentiation were determined by MTT test and alkaline phosphatase activity test. RESULTS AND CONCLUSION:The residual components were the most in the freeze-thaw group, fol owed by the detergent and weak alkal groups, and the least in the trypsin group. Compared with the control group, the absorbance value of cells were lower in the freeze-thaw and detergent groups at days 3, 5, 7 after inoculation (both P<0.05);the alkaline phosphatase activity was lower in the trypsin group at days 5, 7 after inoculation (P<0.05), in the weak alkal group at 7 days after inoculation (P<0.05), and in the detergent group at days 3, 5, 7 after inoculation (P<0.05). Therefore, we can harvest more extracellular matrices by the freeze-thaw method, and the extracellular matrix coating synthesized by the freeze-thaw method is more helpful for cellgrowth than others.关键词
生物材料/骨生物材料/成骨细胞/细胞外基质/脱细胞方法/胰酶法/冻融法Key words
osteoblasts/extracellular matrix分类
医药卫生引用本文复制引用
范利梅,夏荣,窦晓晨..不同脱细胞方式下所得胞外基质存在的差异[J].中国组织工程研究,2014,(30):4863-4867,5.基金项目
安徽医科大学校级科研基金(2012xkj073)生物自组装型表面改性材料的初步构建@@@@the Research Fund of Anhui Medical University, No.2012xkj073 （2012xkj073）
