中国组织工程研究Issue(30)：4873-4877,5.DOI:10.3969/j.issn.2095-4344.2014.30.019
高效液相色谱-示差折光检测法测定辅助生殖培养液中果糖及葡萄糖的含量
Determination of fructose and glucose in assisted reproductive culture medium by high performance liquid chromatography-differential refractive index detector
摘要
Abstract
BACKGROUND:Nowadays, the component content of assisted reproductive culture medium and their test methods are unclear in the quality standards. We need to establish the test methods to fil this vacancy, so as to control the quality of assisted reproductive culture medium effectively. OBJECTIVE:To establish an evaluation method for determination of fructose and glucose in assisted reproductive culture medium, thereby based on which to establish the quality standards. METHODS:High performance liquid chromatography-differential refractive index detector was adopted and Rezex RCM-Monosaccharide Ca2+(8%) (300.0 mm×7.8 mm) column was used. The mobile phase was ultrapure water at the flow rate of 0.6 mL/min and the temperature of 80 ℃. RESULTS AND CONCLUSION:Resolution of the peaks of glucose and fructose was 3.2. The linear ranges of glucose and fructose were 30.1-502 mg/L (r=0.999 8) and 102-408 mg/L (r=0.999 8), respectively. The relative standard deviation of reproducibility test was 0.17%and 0.40%, respectively. The relative standard deviation of stability test was 0.22%and 0.73%, respectively. The glucose group and fructose group average recovery rates were 1.22%and 1.38%, respectively. The methodology of High performance liquid chromatography-differential refractive index detector accorded with the requirements. The glucose contents of samples 1 and 2 were 96.7 mg/L and 99.6 mg/L, respectively. The fructose contents of samples 1 and 2 were 208.5 mg/L and 197.4 mg/L, respectively. A reliable, simple, and accurate method was provided for the quality control of assisted reproductive culture medium, which fil s the domestic vacancy in the quality standards for assisted reproductive culture medium.关键词
生物材料/材料相容性/高效液相色谱法/生物材料基础实验/辅助生殖/培养液/果糖/葡萄糖/示差折光/含量/质量控制Key words
chromatography/quality control/fructose/glucose/research method分类
医药卫生引用本文复制引用
孙雪,黄元礼,柯林楠..高效液相色谱-示差折光检测法测定辅助生殖培养液中果糖及葡萄糖的含量[J].中国组织工程研究,2014,(30):4873-4877,5.基金项目
国家科技支撑计划课题(2012BAI22B01)@@@@the National Key Technology R&D Program of China, No.2012BAI22B01 （2012BAI22B01）
