中国组织工程研究Issue(32)：5091-5096,6.DOI:10.3969/j.issn.2095-4344.2014.32.002
芒果苷对缺氧损伤骨髓间充质干细胞的保护作用
Mangiferin protects bone marrow mesenchymal stem cells against hypoxia
摘要
Abstract
BACKGROUND:Bone marrow mesenchymal stem cells have particularly applied prospects in tissue engineering. However, the death of transplanted cells limits the tissue regeneration. To search a new drug of anti-free radicals and protecting bone marrow mesenchymal stem cells is of great significance. OBJECTIVE:To investigate the protective effects of mangiferin on bone marrow mesenchymal stem cells against hypoxia. METHODS:Rat bone marrow mesenchymal stem cells were cultured in vitro and hypoxia cellmodel was established by cobalt chloride. cells were divided into normal control group, hypoxia group (treated with cobalt chloride), and mangiferin groups (cobalt chloride+20, 40, 80, 160 µmol/L mangiferin). After 12 and 24 hours of hypoxia, superoxide dismutase, malondialdehyde, and catalase levels in the cellsupernatant were determined. After 3, 6, 12, 24 hours of hypoxia, reactive oxygen species change was detected in each group. RESULTS AND CONCLUSION:Mangiferin significantly improved the survival rate of bone marrow mesenchymal stem cells exposed to hypoxia, increased the intracellular superoxide dismutase and catalase activities, decreased intracellular malondialdehyde and reactive oxygen species levels, thereby effectively protecting bone marrow mesenchymal stem cells against hypoxia. These findings indicate that mangiferin has effective protection against hypoxia and strong antioxidant ability, and can significantly reduce oxidative damage.关键词
干细胞/骨髓干细胞/骨髓间充质干细胞/芒果苷/氧化应激/缺氧Key words
bone marrow/mesenchymal stem cells/anoxia/mangifera分类
医药卫生引用本文复制引用
程建文,赵劲民,李晓峰,谭桢..芒果苷对缺氧损伤骨髓间充质干细胞的保护作用[J].中国组织工程研究,2014,(32):5091-5096,6.基金项目
广西卫生厅中医药科技专项(GZKZ 10-011)；广西卫生厅中医药科技专项(GZKZ-Z1104)；高等学校博士学科点专项科研基金(20104503110002)；广西科技厅人口健康与食品安全关键技术研究开发项目(桂科攻1140003A-31)；广西卫生厅自筹经费科研课题(桂卫Z2012058，桂卫Z2012061)；广西教育厅立项项目(桂教201010LX050)@@@@the Special Scientific Fund for Traditional Chinese Medicine in Guangxi Health Bureau, No. GZKZ 10-011, GZKZ-Z1104 （GZKZ 10-011）
the Research Fund for the Doctoral Program of Higher Education, No.20104503110002 （）
Key Technology Research and Development Projects of Population Health and Food Safety of Guangxi Science and Technology Bureau, No. guikegong 1140003A-31 （）
Self-Funded Research Projects of Guangxi Health Bureau, No. guiweiZ2012058, guiweiZ2012061 （）
Projects of Guangxi Education Bureau, No. guijiao201010LX050 （）
