中国组织工程研究Issue(32)：5120-5125,6.DOI:10.3969/j.issn.2095-4344.2014.32.007
γ型干扰素增强脂肪干细胞介导的免疫抑制
Interferon-gamma enhances immunosuppression mediated by adipose-derived stem cells
摘要
Abstract
BACKGROUND:Adipose stem cells can differentiate into bone cells, cartilage cells, smooth muscle cells, myocardial cells and nerve cells. Adipose-derived stem cells and mesenchymal stem cells have very similar biological properties, and they are also very close in terms of cellsurface marker expression profile. OBJECTIVE:To investigate the expression of human leukocyte antigen II in adipose-derived stem cells and its immunogenicity and immunomodulatory effect, and to explore the specific mechanism. METHODS:After isolation and culture, the adipose-derived stem cells were stimulated with interferon-γ, and the expression of human leukocyte antigen II was detected by flow cytometry, the expression of indoleamine 2,3-dioxygenase was determined by real-time PCR and western blot. Adipose-derived stem cells were used to stimulate lymphocytes, and the cellproliferation was observed. The lymphocytes were stimulated with phytohemagglutinin, and then 1×102-1×105 adipose-derived stem cells were added to inhibit the proliferation;meanwhile, adipose-derived stem cells, a third party, at a dose of 1×102-1×105 were added to the ongoing two-way mixed lymphocyte reaction, and the cellnumber was calculated as count per minute. RESULTS AND CONCLUSION:The expression of human leukocyte antigen II and indoleamine 2,3-dioxygenase was low in adipose-derived stem cells, but up-regulated with stimulation of interferon-γ. Adipose-derived stem cells could not stimulate lymphocyte proliferation, but could inhibit lymphocyte proliferation induced by phytohemagglutinin or al ogeneic lymphocytes, and interferon-γwas highly expressed in the supernatant of co-culture of adipose-derived stem cells and mixed lymphocytes. These results indicate that the adipose-derived stem cells have low immunogenicity and can inhibit cellproliferation of lymphocytes in vitro, and the addition of exogenous interferon-γcan enhance the immunosuppressive effects of adipose-derived stem cells.关键词
干细胞/脂肪干细胞/免疫抑制/脂肪源性干细胞/吲哚胺-2,3-双加氧酶/植物血凝素/白细胞抗原Ⅱ/淋巴细胞增殖/混合淋巴细胞反应/γ型干扰素/新疆维吾尔自治区自然科学基金Key words
adipocytes/lymphocytes/immune tolerance/interferon-gamma/phytohemagglutinins分类
医药卫生引用本文复制引用
依里牙尔·依里哈木,王云海,王理,许新才,艾木拉古丽·阿布拉,张文斌..γ型干扰素增强脂肪干细胞介导的免疫抑制[J].中国组织工程研究,2014,(32):5120-5125,6.基金项目
新疆医科大学第一附属医院组织工程专项项目(201022GC02)；新疆维吾尔自治区自然科学基金资助项目(2014211C036)；国家临床重点专科-普通外科专业建设项目@@@@the Tissue Engineering Special Fund of the First Affiliated Hospital of Xinjiang Medical University, No.201022GC02 （201022GC02）
the Natural Science Foundation of Xinjiang Uygur Autonomous Region, No.2014211C036 （）
the National Key Clinical Specialty：the Project of General Surgery （）
