中国组织工程研究Issue(36)：5825-5829,5.DOI:10.3969/j.issn.2095-4344.2014.36.015
脉络宁对体外模拟体内生理环境寄养断肢系统缺血/再灌注损伤模型的作用
Effect of Mailuoning on an ischemia/reperfusion injury model in the system of simulated in vivo physiological environment for amputated limbs
摘要
Abstract
BACKGROUND:Reducing the ischemia-reperfusion injury in the system of simulated in vivo physiological environment for amputated limbs, can extend the preservation time and improve the replantation success rate of amputated limb. OBJECTIVE:To investigate the effect of Mailuoning on the ischemia-reperfusion injury in the system of simulated in vivo physiological environment for amputated limbs. METHODS:The left hind legs harvested from 18 healthy adult male Bama mini-pigs were randomly divided into three groups:cold storage group, blood perfusion group, Mailuoning combined blood perfusion group, with six pigs in each group. After the left hind legs of each pig in al groups were amputated and stored at room temperature for 3 hours, the amputated legs were placed at 4 ℃, perfused with blood, or perfused with Mailuoning combined blood. After 6 hours of perfusion, the morphology of amputated limbs was observed under transmission electron microscope. The mRNA levels of apoptosis proteins Caspase3 and inflammatory factor interleukin-1βwere detected by real time fluorescent quantitative RT-PCR.RESULTS AND CONCLUSION:Transmission electron microscopy results showed that, the muscle fibers in blood perfusion group and Mailuoning combined blood perfusion group arranged more orderly and were more complete than cold storage group, the swel ing of mitochondria was lighter. In addition, the condition in Mailuoning combined blood perfusion group was better than in blood perfusion group. RT-PCR results showed that the expression of Caspase3 and interleukin-1βwas increased in al groups;at the same time, the expression level in blood perfusion group and Mailuoning combined blood perfusion group was significantly lower than those in cold storage group. Mailuoning combined blood perfusion group had s lower expression than blood perfusion group. In the system of simulated in vivo physiological environment for amputated limbs, Mailuoning can significantly reduce the ischemia/reperfusion injury of skeletal muscle cells and prolong the preservation time of amputated limbs.关键词
实验动物/组织构建/脉络宁/断肢/体外寄养/模拟人体生理环境/缺血再灌注/低温保存Key words
drug, Chinese herbal/hindlimb/cryopreservation/reperfusion injury分类
医药卫生引用本文复制引用
张绍春,秦新愿,左有为,高磊,尹叶锋,王江宁..脉络宁对体外模拟体内生理环境寄养断肢系统缺血/再灌注损伤模型的作用[J].中国组织工程研究,2014,(36):5825-5829,5.基金项目
首都临床特色应用研究资助项目(Z121107001012113)Funding:the Capital Featured Clinical Research Project, No. Z121107001012113 （Z121107001012113）
