中国组织工程研究Issue(37)：5929-5934,6.DOI:10.3969/j.issn.2095-4344.2014.37.005
中频脉冲电磁场照射骨髓间充质干细胞的拉曼光谱
Raman spectroscopy of bone marrow mesenchymal stem cells in medium-frequency pulsed electromagnetic fields
崔向荣 1苏伟 1吴智辉 1孟令晶 1黄钊 1覃万安1
作者信息
- 1. 广西医科大学第一附属医院创伤骨科手外科，广西壮族自治区南宁市 530021
- 折叠
摘要
Abstract
BACKGROUND:Studies about low-frequency pulsed electromagnetic fields interfering with bone marrow mesenchymal stem cells proliferation and differentiation are many, but the Raman spectra of single stem cells irradiated in electromagnetic fields analyzed by surface Raman spectroscopy analysis are rarely reported. OBJECTIVE:To compare the difference in Raman spectra of bone marrow mesenchymal stem cells with or with no irradiation of 3 000 Hz pulsed electromagnetic fields. METHODS:Bone marrow mesenchymal stem cells isolated from Sprague-Dawley rats were cultured and identified. Passage 3 cells were inoculated into 6-wel plates and divided into two groups:pulsed electromagnetic field irradiation group and blank control group. After cultured for 7 days, cells in the two groups were transferred to physiological saline, and 30 cells were randomly col ected from each group. Four Raman spectra were harvested from each celland the average relative intensity of Raman spectra was calculated and compared between two groups. RESULTS AND CONCLUSION:There were the same Raman peaks in the two groups, and the waveforms were basical y same in the two group based on the curve mapping by origin 7.0 software. The peak value in the irradiation group was decreased compared with the blank control group. Laser optical tweezers Raman spectroscopy can be applied to study the biochemical changes of a single stem cellat the molecular level. The Raman spectra of bone marrow mesenchymal stem cells irradiated by 3 000 Hz pulsed electromagnetic fields differ from those without irradiation, and the peak also lowered after irradiation.关键词
干细胞/骨髓干细胞/拉曼光谱/骨髓间充质干细胞/中频/脉冲电磁场/光镊/干细胞图片文章Key words
stem cells/mesenchymal stem cells/electromagnetic fields/optical tweezers分类
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崔向荣,苏伟,吴智辉,孟令晶,黄钊,覃万安..中频脉冲电磁场照射骨髓间充质干细胞的拉曼光谱[J].中国组织工程研究,2014,(37):5929-5934,6.
