中国组织工程研究Issue(37)：5988-5992,5.DOI:10.3969/j.issn.2095-4344.2014.37.015
转染血管内皮生长因子基因的骨髓间充质干细胞修复糖尿病大鼠足背创面
Transplantation of bone marrow mesenchymal stem cells transfected by vascular endothelial growth factor gene promotes foot wound healing in diabetic rats
摘要
Abstract
BACKGROUND:Diabetic foot ulcers threaten the patients’ health and even survival seriously. It is an international difficult problem and lacks an effective treatment. But gene therapy and stem celltherapy possess special advantages and potential in wound healing. OBJECTIVE:To assess the therapeutic effect of transplantation of bone marrow mesenchymal stem cells transfected by human vascular endothelial growth factor 165 (hVEGF165) gene on foot wound healing in diabetic rats. METHODS:Recombinant adenovirus was established in vitro which expressed hVEGF165 gene and transfected into the third generation of bone marrow mesenchymal stem cells. Total y 120 male Wistar rats were divided into five groups:group A (non-diabetic controls), group B (diabetic controls), group C (Ad-hVEGF165 therapy), group D (stem celltherapy) and group E (transplantation of bone marrow mesenchymal stem cells transfected by Ad-hVEGF165 gene). Rats in the latter four groups were intraperitoneal y injected with streptozotocin to induce diabetic models. In al rats, a 3 mm×7 mm rectangular ful-thickness skin sample was cut from the instep of the hind foot to make a model of foot wound. The rats were subcutaneously injected at equidistant six points 5 mm distal to the wound edge on the dorsum of the foot:50μL PBS per point for group A, 50μL adenovirus suspension (1×1013 pfu/L) per point for group C, 50μL stem cellsuspension (1×1010/L) per point for group D, and 50μL adenovirus suspension+50μL stem cellsuspension per point for group E. RESULTS AND CONCLUSION:After injection, the rate of wound healing, the expression of VEGF and the qualities of capil aries in group E were higher when compared with groups B, C, D (P<0.05), but were lower than those in group A (P<0.05). Transplantation of bone marrow mesenchymal stem cells transfected by hVEGF165 gene can promote foot wound healing, angiogenesis and expression of VEGF in diabetic rats.关键词
干细胞/移植/血管内皮生长因子/干细胞移植/基因/腺病毒/骨髓间充质干细胞/糖尿病/糖尿病足/创面修复/血管生成/甘肃省自然科学基金Key words
stem celltransplantation/diabetic foot/neovascularization, pathologic/mesenchymal stem cells分类
医药卫生引用本文复制引用
蔡黔,万江波,梁文佳,刘毅..转染血管内皮生长因子基因的骨髓间充质干细胞修复糖尿病大鼠足背创面[J].中国组织工程研究,2014,(37):5988-5992,5.基金项目
甘肃省自然科学基金(1208RJZA155)；兰州军区医药卫生科研面上项目(CLZ11JB07) （1208RJZA155）
